| 摘要: |
| 应用胶体金免疫层析技术,采用柠檬酸三钠还原法制备25nm的胶体金颗粒,标记抗犬细小病毒单克隆抗体CPV-F1株后包被于玻璃纤维膜作为金标垫,在硝酸纤维素膜上分别包被羊抗小鼠IgG二抗和抗犬细小病毒单克隆抗体CPV-B6株作为质控线和检测线,将吸水垫、硝酸纤维素膜、金标垫和样品垫分别粘贴于背衬板上制成犬细小病毒抗原胶体金检测试纸条。该试纸条具有良好的特异性和敏感性,与韩国进口犬细小病毒检测试纸产品对比,总符合率为98%,整个检测过程只需10min,具有操作简单,快速,结果直观易辨的优点。 |
| 关键词: 犬细小病毒 抗原 胶体金试纸条 |
| DOI： |
| 投稿时间：2013-12-17修订日期：2014-02-25 |
| 基金项目: |
|
| Study of Colloidal Gold Strip in Detecting the Canine Parvovirus |
|
| (WuHan Chopper Biological Co.,Ltd.) |
| Abstract: |
| Based on the principle of colloidal gold immunochromatography, with the tri-sodium citrate reduction method, colloidal gold strip was prepared and applied to detect the canine parvovirus. Anti-CPV Monoclonal antibody and goat anti-mouse IgG were coated on the test region and control zone respectively. The accuracy of stip is 98% compared to the strip imported from Korea. The whole experimentation merely needed ten minutes. This test shows that this strip has the merits of convenient operation, celerity and intuitional. |
| Key words: canine parvovirus antigen immunogold labeling strip |
