| 摘要: |
| 为建立易悬浮驯化和对流感病毒易感的MDCK细胞株，将表达siat7e和st3galI基因的真核表达载体电击转染MDCK细胞，采用有限稀释法、荧光定量PCR和流式细胞术成功筛选到1株双基因稳定共表达MDCK-C5细胞株 ， 为流感疫苗工业化大规模生产奠定了基础。 |
| 关键词: MDCK细胞 siat7e基因 st3gal I基因 |
| DOI： |
| 投稿时间：2013-12-24修订日期：2014-01-22 |
| 基金项目:国家自然科学基因项目(31101817)和“十一五”国家科技支撑计划重点项目(2009BADB4B00) |
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| The Construction of MDCK Cell Line Co-expressing Siat7e and St3gal I Gene |
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| (China Institute of Veterinary Drug Control,Beijing,100081 China) |
| Abstract: |
| In order to obtain MDCK cell which can adapt to suspension culture and be susceptible to influenza virus，a plasmid pReceiver-siat7e-st3galⅠwas transfected into MDCK cell line. A new cell line MDCK-C5 co-expressing siat7e and st3galⅠstably was selected by limiting dilution, quantitive PCR, and flow cytometry. The data established foundation for industrialized large scale production of influenza vaccine. |
| Key words: siat7e gene st3gal I gene MDCK cell |
