| 摘要: |
| [摘要 ]建立超高效液相色谱-串联质谱法，并对克伦特罗（Clenbuterol）在绒山羊体内残留消除规律进行了研究。选取60只健康绒山羊（30 4.8 kg），平均分成2组，分别在饲料中添加150μg/kgBW（A组）和300μg/kgBW（B组）克伦特罗，连续饲喂25d后停药。建立UPLC-MS/MS检测方法，监测喂药期尿液、休药期尿液和组织中克伦特罗含量。 克伦特罗在饲喂期间，A组尿液中平均含量为289-5313μg/L，B组尿液中平均含量为1323-11378μg/L。停药后尿液中克伦特罗含量迅速下降，停药40d后，A、B两组均下降至10μg/L以下。停药后，取绒山羊肝脏、肾脏、肺脏、肌肉测定其残留量。结果表明，A、B两组停药后组织中克伦特罗含量持续下降，停药40d后,肝脏含量33μg/kg，肾脏约5μg/kg，其余组织均未检出。 |
| 关键词: 绒山羊 克伦特罗 超高效液相色谱-串联质谱法 消除 残留量 |
| DOI： |
| 投稿时间：2014-01-10修订日期：2014-02-18 |
| 基金项目: |
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| Studies on the determination of clenbuterol depletion in Cashmere goats by UPLC-MS/MS |
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| (Animal feed quality and safety of venterinary testing centre in Liaoning province Shenyang China) |
| Abstract: |
| Abstract: The depletion of clenbuterol in urine and tissues after treatment with clenbuterol were studied with the developed UPLC/MS/MS method. 60 goats（30 4.8 kg）were divided into 2 groups named group A and B .Goats were fed 150μg/kgBW（ group A）and 300μg/kgBW（ group B） for twenty five consecutive days.Samples of urine and tissues were collected to determine clenbuterol concentration by UPLC-MS/MS. The results showed that the mean concentrations of clenbuterol were 289-5313μg/L and 1323-11378μg/L in group A and B in urine during feeding period. The concentrations of clenbuterol in urine were rapidly decreasd under 10μg/L during the withdrawal period . With a 40-day withdrawal period, the mean concentrations of clenbuterol were 33μg/kg in liver and 5μg/kg kidney, but the concentration of muscle and lung were not detected. |
| Key words: Cashmere goats clenbuterol UPLC-MS/MS depletion residues |
