| 摘要: |
| 建立检测健胃散等3种中兽药散剂中违规添加氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星等4种喹诺酮类药物的HPLC-PDAD法。采用Atlantis T 3(十八烷基硅烷键合硅胶,4.6mm i.d×250mm,5μm)；流动相为磷酸溶液∶乙腈∶甲醇(85.5∶7.0∶7.5),流速为1.0 mL.min-1；检测波长：283nm。4种喹诺酮类药物检查方法的线性关系良好,r>0.9999；回收率均在98.4% ～104.6%范围内,RSD均在0.9%～2.3%之间；4种喹诺酮类药物在3种中兽药散剂中的检出限均为200mg/kg。该方法简便、快速、准确,可用于中兽药散剂中违规添加喹诺酮类药物的检测。 |
| 关键词: 中兽药散剂 氧氟沙星 诺氟沙星 环丙沙星 恩诺沙星 反相高效液相色谱法。 |
| DOI： |
| 投稿时间：2014-01-26修订日期：2014-03-18 |
| 基金项目:中国兽医药品监察所所级科研项目：兽药高效液相色谱图库研究和建立（200806） |
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| Determination of four Quinolones Illegally Adulterated into Three Traditional Chinese Veterinary Medicine Powders by HPLC |
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| (China Institution of Veterinary Drug Control) |
| Abstract: |
| To establish a HPLC-PDAD method for determination of four quinolones in three traditional Chinese veterinary medicine powders．Samples were extracted with a mixture of a solution of 2%phosphoric acid and acetonitrile(1:1), RP-HPLC-PDAD was performed on a column of Atlantis T 3 (4.6 mm×250 mm, 5 μm) at 283nm. The mobile phase consisted of a mixture of a solution of phosphoric acid, acetonitrile and methanol(85.5:7.0:7.5) at a flow rate of 1.0 mL.min-1. In the linear range the correlation coefficients over 0.9999．The average recoveries were 98.4% ~104.6% with RSD between 0.9%~2.3%. This method is rapid, simple and accurate. It can be used in the determination of Quinolones illegally adulterated into the traditional Chinese veterinary medicine powder． |
| Key words: HPLC, PDAD, Ciprofloxacin, Ofloxacin, Norfloxacin, Enrofloxacin, Traditional Chinese Veterinary Medicine Powder. |
