| 摘要: |
| 为了建立高效液相色谱法测定尼卡巴嗪的含量，采用Waters X-Bridge C18(150 mm×4.6 mm,5 μm)色谱柱，乙腈-水(用磷酸调pH至3.0)为流动相，梯度洗脱，流速为1.0 mL·min-1，检测波长0~5 min为297 nm，5 min开始为350 nm，柱温30 ℃，进样体积为20 μL。2-羟基-4,6-二甲基嘧啶(HDP)的回归方程为：y=23452x-456，相关系数r=0.99999，4,4’-二硝基均二苯脲(DNC)的回归方程为：y=102388x+5296，相关系数r=0.9999，即HDP在0.29~29.12 μg/mL范围内具有良好的线性， DNC在0.71~70.90 μg/mL范围内具有良好的线性。该方法简便、准确、灵敏度高、重复性好，可以通过同时测定尼卡巴嗪中HDP和DNC来计算尼卡巴嗪的含量。 |
| 关键词: 尼卡巴嗪 HDP DNC 高效液相色谱法 |
| DOI： |
| 投稿时间：2014-02-17修订日期：2014-04-25 |
| 基金项目: |
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| Study on the Determination of Nicarbazine by HPLC |
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| (Henan Provincial Supervising Agency of Veterinary Drugs,Henan) |
| Abstract: |
| To develop the method of HPLC for determination of nicarbazine. The liquid chromatography separation was carried on XBridge C18 column (150 mm×4.6 mm,5 μm) with acetonitrile-water(pH was adjusted to 3.0 by H3PO4) as mobile phase under the column temperature 30 ℃.The detection wavelength was 297 nm at 0~5 min,and 350 nm after 5 min, and the injection quantity was 20 μL .The equation of HDP was y=23452x-456, r=0.99999； The equation of DNC was y=102388x 5296,r=0.9999, the linear range of HDP and DNC were 0.29~29.12 μg/mL and 0.71~70.90 μg/mL respectively. The method was simple , accuracy,and with high sensitivity.The content of nicarbazine can be calculated by determining HDP and DNC at the same time. |
| Key words: nicarbazine HDP DNC HPLC |
