| 摘要: |
| 为寻求抗ALV-J感染雏鸡用药物，通过CCK-8试剂盒检测细胞活力以确定利巴韦林、病毒灵和黄芪多糖对DF1细胞的最大安全浓度，用ELISA试剂盒检测ALV-J P27抗原表达量，以确定上述抗病毒药物体外抗ALV-J活性。结果显示，利巴韦林、病毒灵和黄芪多糖对DF1细胞的最大安全浓度分别为0.0391、0.0625和0.0625 mg/mL。在安全范围内，利巴韦林对P27的表达量具有明显的抑制作用,黄芪多糖在0.0039~0.0625 mg/mL安全浓度范围内明显抑制P27的表达，病毒灵在安全范围内对P27的表达基本上没有抑制作用。试验表明，利巴韦林和黄芪多糖对ALV-J的增殖有明显的抑制作用，有望作为该病净化的辅助性防制药物。 |
| 关键词: 利巴韦林 病毒灵 黄芪多糖 ALV-J P27 |
| DOI： |
| 投稿时间：2014-02-25修订日期：2014-04-27 |
| 基金项目:公益性行业(农业)科研专项 (201203055) |
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| The Activity Experiment of Ribavirin, Moroxydine and Astragalan Against ALV-J |
|
| (Animal Science and Technology of Shan Dong Agricultural University) |
| Abstract: |
| To acquire the anti-ALV-J drug，P27 expression quantity influence of Ribavirin, moroxydine and astragalan on ALV-J (NX0101) are researched by the CCK 8 kits cell detected for the maximum safe concentrations of the three antiviral drugs on DF1 cell and ALV-J P27 antigen ELISA test kit for P27 expression quantity.The results show that the maximum safe concentrations of Ribavirin, moroxydine and astragalan on DF1 cell are 0.039, 0.0625 and 0.0625 mg/mL. The obvious inhibitory concentration of Ribavirin for the P27 expression quantity in the the scale of safe concentrations is 0.0391 mg/mL. The concentration range of astragalan inhibiting the expression of ALV-J P27 are 0.0039~0.0625 mg/mL. However, varied concentration of moroxydine in the research has no inhabitory effect on P27 expression quantity. The aforementioned results show that ribavirin and astragalan can inhabit the propogation of ALV-J, providing a perfect prospect for the ALV-J eradication in chinken in the future. |
| Key words: ribavirin moroxydine astragalan ALV-J P27 |
