| 摘要: |
| 建立了猪肉、猪肝和猪尿中阿托品残留的超高效液相色谱-串联质谱(UPLC-MS/MS)的检测方法。样品经乙腈-0.1%甲酸水(V/V,90:10)溶液提取,Agela Cleanert? PEP-2固相萃取柱净化后,采用UPLC-MS/MS测定,外标法定量。结果表明,猪组织及猪尿中阿托品的检测限为0.2 μg/kg,定量限为0.5 μg/kg。在0.5～2.5 μg/kg添加浓度范围内阿托品的平均回收率78.3%～98.2%之间,批内、批间相对标准偏差均小于15％。该方法简便、快速、灵敏度高、重现性好,适用于猪组织及猪尿中阿托品的含量测定。 |
| 关键词: 猪组织 猪尿 阿托品 残留 超高效液相色谱-串联质谱 |
| DOI： |
| 投稿时间：2014-06-10修订日期：2014-07-15 |
| 基金项目: |
|
| Determination of Atropine in Porcine Muscle,Liver and Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
|
| (Zhengzhou University) |
| Abstract: |
| A method based on ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was developed for determination of atropine in porcine muscle,liver and urine. The analyte was extracted with acetonitrile-0.1% formic acid in water (V/V,90:10),and was cleaned up by the Agela Cleanert? PEP-2cartridge. Quantification of the analyte was achieved by UPLC-MS/MS with multiple reaction monitoring (MRM) using external standard method. The results indicated that the detection limits and quantification limits of the method were 0.2 μg/kg and 0.5 μg/kg. The average recoveries of the drugs ranged from 78.3%～98.2% at spiked levels of 0.5~2.5 μg/kg with RSD less than 15%. The method was convenient,rapid,sensitive and reproducibility for monitoring atropine residues in porcine muscle,liver and urine。 |
| Key words: porcine tissues porcine urine atropine residue UPLC-MS/MS |
