| 摘要: |
| 就细胞的冷冻密度和复苏温度对细胞活力的影响做了分析。取处于对数生长期的细胞以0.5x107～4.0x107 /mL之间的4个细胞密度分别进行冷冻,2周之后复苏细胞,检验其复苏活力;又分别在37 ℃、38 ℃和39 ℃的水温复苏同一批细胞，复苏后检验细胞的活力。结果表明，随着冷冻密度的升高，复苏活力逐渐下降；在38 ℃水温复苏细胞的平均活力比在37 ℃和39 ℃水温复苏细胞的平均活力高，而且稳定。因此，细胞的冷冻密度和复苏活力之间存在显著负相关性，38 ℃的水温更适于复苏细胞。 |
| 关键词: BHK21细胞 冷冻密度 复苏温度 细胞活力 |
| DOI： |
| 投稿时间：2014-06-20修订日期：2014-09-17 |
| 基金项目: |
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| Effect of Cells’ Density Frozen and Recovery Temperature on BHK21 Cells’ Viability |
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| (JIN YU BAO LING Bio-pharmaceuticals Company,LTD,Hohhot,010030) |
| Abstract: |
| The experiment analyzed the relationship among frozen density , recovery temperature and cells’ viability. We respectively frozen logarithmic growth phase cells in four different cell density between 0.5 X107 cells/ ml～4.0 X107 cells / ml .After two weeks, We revived the cells we frozen and then tested their viability ;We revived the same cells In the warm water of 37 ℃，38 ℃ and 39 ℃ respectively. After the recovery, the viability of the cells were tested . The experiment showed when the frozen density was higher, the cell viability was lower. And the average viability of the cells revived in 38 ℃ was higher and more stable than the cells revived in 37 ℃ and 39 ℃ . Therefore, there was a significant negative correlation between frozen density and cells’ recovery viability, and the warm water of 38 ℃ was suitable for reviving cells. |
| Key words: BHK21 cell,frozen density recovery temperature cells’ viability |
