| 摘要: |
| 本项目分别对连翘进行不同方法的提取,得到其产物,再进行实验动物给药并制备含药血清,最后利用HPLC方法测定其含量。色谱条件：色谱柱PT-230AColume heater Apollo C18柱(150mm×4.6mm,5μm),流动相为乙腈-水 25：75,流速0.8ml/min,检测波长为235nm,柱温25 ℃。结果显示：煎剂组(B组)和粗粉组(C组)在5个时间点都测不出连翘苷的含量,提取物组(A组)只有在1.5小时可以测到含量极低的连翘苷,连翘苷纯品组(D组)在0.5、1、1.5、2、4小时的连翘苷含量分别为：0.205mg/ml, 0.266mg/ml、0,099mg/ml、0.046mg/ml、0 mg/ml。 |
| 关键词: 连翘苷 含药血清 制备 测定 |
| DOI： |
| 投稿时间：2014-07-16修订日期：2014-09-02 |
| 基金项目:山西省农科院科技攻关项目(2012YGG33)；山西省科技攻关项目(20120311023-3)；山西省科技攻关项目(20140311019-1) |
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| Preparation and determination of forsythin medicated serum |
| 任杰 |
| (institute of animal husbandry and veterinry,shanxi academy of agricultural sciences) |
| Abstract: |
| This project was extracted separately forsythia different methods to get their product, then experimental animals to medicine and the preparation of medicated serum, final determination of its content using HPLC methods. Chromatographic conditions: Column PT-230AColume heater Apollo C18 column (150mm × 4.6mm, 5μm), mobile phase of acetonitrile - water 25:75, flow rate 0.8ml/min, the detection wavelength was 235nm and the column temperature 25 ℃. The results showed that: decoction group (group B) and coarse (group C) at five time points undetectable levels forsythin, extract group (A) can be measured only 1.5 hours to very low levels the phillyrin, forsythin pure group (group D) were in 0.5,1,1.5,2,4 hours forsythin content: 0.205mg/ml, 0.266mg/ml, 0,099 mg / ml, 0.046 mg / ml, 0 mg / ml. |
| Key words: Phillyrin containing serum preparation determination |
