| 摘要: |
| 为有效控制鸡大肠杆菌病，对不同省区鸡源大肠杆菌致病菌株的分布情况及耐药特征进行了调查分析，分别采用PCR法和MIC法进行了系统进化群分群检测和药敏试验，采用皮下注射进行了动物致病性试验，并采用SPSS17.0软件进行差异显著性分析。结果显示：低致病群B1（38.13%）和非致病群A（34.02%）菌株分布相对较多，其次是高致病群D（21.23%）和B2（6.16%）；鸡源大肠杆菌耐药严重，12种药物的耐药率在50%以上，多重高耐药问题突出，多重耐药率高达98.86%；不同省区鸡源大肠杆菌菌株的耐药程度存在差异，个别药物（如奥格门丁、头孢噻呋等）差异显著；高致病群B2和D群菌株的耐药程度较B1和A群菌株严重，其中氧氟沙星、庆大霉素、大观霉素、头孢噻呋、磺胺异噁唑、复方新诺明、奥格门丁等7种药物差异显著（P<0.05）。试验表明，我国鸡源大肠杆菌高致病菌株检出率高，且耐药程度相对严重，在适宜条件下更易导致鸡群发病且较难控制。 |
| 关键词: 鸡大肠杆菌 系统进化群 致病性 耐药性 |
| DOI： |
| 投稿时间：2014-07-17修订日期：2014-08-25 |
| 基金项目:“十二五”科技基础性工作专项(项目编号：2012FY111000) |
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| Antibiotical Resistance Investigation and Phylogenetic Groups Analysis of Chicken Originated E.coli Strains from Different Province |
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| (China Animal Health and Epidemiology Center,Qingdao Shandong) |
| Abstract: |
| In order to prevent and control chicken colibacillosis effectively, the distribution and drug-resistance of pathogenic poultry originated E.coli strains from different province were investigated and analyzed. The phylogenetic groups and antimicrobial susceptibility was detected by PCR and MIC method respectively; the animal pathogenicity test was carried out by subcutaneous injection; and the significance of difference was analyzed by SPSS17.0. Results showed that the lowly pathogenic group B1(38.13%) and the nonpathogenic group A(34.02%) distributed more than the highly pathogenic group D(21.23%) and B2(6.16%). The resistance of chicken originated E.coli was serious, the resistant rate of 12 kinds of drugs was more than 50.0%, and multiple drug resistance problems was serious, multiple resistant rate was as high as 98.86%. The resistance of E.coli stains from different province was different, and there was significant difference between dividual drugs(e.g. amoxycillin/clavulanic acid and ceftiofur). The resistance of the strains from highly pathogenic group group B2 and D was higher than the strains from group B1 and A, and there was significant difference for 7 kinds of drugs such as OFL, GM, SPT, EFT, SF, SXT, A/C(P<0.05). It conclude that the ratio of highly pathogenic strains from chicken originated E.coli in China is higher, and the drug-resistance of that is relatively serious, which are likely to lead to poultry disease and difficult to control under the suitable conditions. |
| Key words: chicken originated E.coli phylogenetic groups pathogenicity drug-resistance |
