| 摘要: |
| 建立了17种兽药中非法添加氨茶碱和二羟丙茶碱的高效液相色谱法,以十八烷基键合硅胶为填充剂,0.025mol/L磷酸二氢钾溶液-甲醇为流动相,二极管阵列检测器全波长(200～400nm)扫描,270nm进行测定,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。在此液相色谱条件下,氨茶碱和二羟丙茶碱与其他物质峰能很好的分离,两种药物在0.5～200μg/m范围内线性关系良好,相关系数r均为1.0000。按外标法以峰面积计算,氨茶碱和二氢丙茶碱的平均回收率为92.6%～108.3%,RSD为0.05%～3.7%。结果表明,该方法适合兽药中氨茶碱和二羟丙茶碱的检测。 |
| 关键词: 氨茶碱 二羟丙茶碱 高效液相色谱法 峰纯度检查 光谱相似度检查 |
| DOI： |
| 投稿时间：2014-07-27修订日期：2014-10-09 |
| 基金项目:河南省重点科技攻关计划(编号132102110078) |
|
| Determination of Aminophylline and Diprophylline in 17 kinds of veterinary drug by HPLC-PDA |
|
| (Henan Institute of Veterinary Drug Control) |
| Abstract: |
| A method for the determination of Aminophylline and Diprophylline in 17 kinds of veterinary drug was developed by the high performance liquid chromatography with photo-diode array detector (HPLC-PDA). It was tested with C18 column, using 0.025mol/L potassium dihydrogen phosphate solution and methanol as the mobile phase. The detection wavelength was set at 270 nm and simultaneously collected the spectrum at wavelength of 200～400nm. Peak purity test and spectrum similar test were helped to identify the Aminophylline and Diprophylline. The concentration of the Aminophylline and Diprophylline in the range of 0.5 μg/mL～200 μg/mL showed a good linear relationship, r=1.0000. The average recoveries of the drugs ranged from 92.6%～108.3% at spiked levels with RSD less than 3.7%. The results showed that the method was suitable for detection of Aminophylline and Diprophylline in veterinary drug. |
| Key words: aminophylline diprophylline HPLC method peak purity test spectrum similar test |
