| 摘要: |
| 为了解吉林省长春地区猪源大肠杆菌的耐药情况，于2013年采集318份猪源样品，分离鉴定大肠杆菌275株。以氨苄西林、头孢噻肟等15种药物进行了药物敏感性实验，多重 PCR 方法进行系统进化分群。结果表明，大肠杆菌分离株对四环素、氨苄西林和磺胺甲基异恶唑耐药最严重（83.63%、52.72%、51.27%），全部菌株对美洛培南、多粘菌素敏感，其中176 株菌表现为对3类以上抗生素的多重耐药（64.00%）。从仔猪腹泻样品分离大肠杆菌对β-内酰胺类抗生素、喹诺酮类抗生素、四环素、氯霉素及磺胺甲基异恶唑的耐药率显著高于健康猪和猪肉样品分离株的耐药率。大肠杆菌分离株主要为 A 群和 B1群。研究获得了吉林长春地区猪源大肠杆菌耐药性的基本流行病学数据，为指导养殖业的临床用药及耐药性监测提供了依据。 |
| 关键词: 猪 大肠杆菌 耐药性 |
| DOI： |
| 投稿时间：2014-08-12修订日期：2014-09-16 |
| 基金项目:国家科技重大专项（No.2013ZX10004-217-002）；863项目（2012AA022006）；广州市科技计划项目（No.201300000036） |
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| Antimicrobial Resistance of Escherichia coli Isolated from Swine in Changchun |
| 王基伟 |
| (jilinnongyedaxue dongwukexuejishuxueyuan) |
| Abstract: |
| In order to investigate the antimicrobial resistant of Escherichia coli from swine, a total of 275 Escherichia coli were recovered from 318 swine origin samples in Chuangchun in Jilin province, The phylogenetic background determinated by multiplex PCR. Susceptibility of 275 isolates to fifteen antimicrobial agents were tested. Among swine E. coli isolates, 83.63% were resistant to tetracycline, 52.72% to ampicillin and 51.27% to sulfamethoxazole. Most of the isolates (176) were multiple resistant bacteria strains; it meant that 64% of the E. coli strains were resistant to more than 3 kinds of antimicrobial agents. All of the isolates were sensitive to meropenem and polymyxin B. In addition, the drug-resistant rates to quinolones, tetracycline, chloramphenicol, sulfanilamide, and β-lactam antibiotics of the E. coli isolates from diarrhea piglets are remarkbly higher than those isolates from healthy swine and pork. The majority of isolates belonged to phylogenetic group A and B1. In this study, epidemiological background and basic data of drug resistance of E. coli from swine in Chuangchun in Jilin province. It could give some directions to clinical medication and drug resistance surveillance. |
| Key words: swine Escherichia coli drug resistance |
