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长春市市售猪肉鸡肉中金黄色葡萄菌和沙门氏菌分离及耐药性分析
王莹,陈萍
0
(吉林农业大学食品科学与工程学院)
摘要:
目的:对肉品中金黄色葡萄球菌及沙门氏菌进行耐药分析,为其风险评估提供依据。 方法:采集吉林省长春市市售猪肉、鸡肉样品,按照国标方法分离金黄色葡萄球菌、沙门氏菌,利用K-B 法(CLSI)药敏试验检测金黄色葡萄球菌和沙门氏菌对头孢噻肟钠,左旋氧氟沙星、庆大霉素、四环素4种抗生素的耐药性。 结果:共分离出金黄色葡萄球菌46株,检出率为46%;沙门氏菌48 株,检出率为16.84%。金黄色葡萄球菌对4种抗生素的耐药率在0%~56.52%,共产生6种耐药谱。沙门氏菌对4种抗生素的耐药率在16.67%~87.5%,共产生9 种耐药谱。 结论:长春市肉品中金黄色葡萄球菌、沙门氏菌耐药现象普遍。
关键词:  金黄色葡萄球菌  沙门氏菌  耐药性
DOI:
投稿时间:2014-08-12修订日期:2014-09-01
基金项目:国家科技部重大专项(2013ZX10004217-002)
Isolation and Drug Resistance Analysis of S.aureus and Salmonella spp. from Retail Chicken and Pork in Changchun
(College of Food Science and Engineering,Jilin Agriculture University)
Abstract:
Abstract: Objective: This study aimed at updating knowledge on the antimicrobial resistance characteristics of S.aureus and Salmonella spp. from chicken and pork in Changchun. The results collected during this study provide the first baseline data for risk assessment. Methods: Chicken and pork samples were collected in Changchun. S.aureus and Salmonella spp. were isolated according to National Food Safety Standard. The susceptibility to CEX,GEN,LVX and TET was performed according to the disk diffusion testing. Results: 46 S.aureus and 48 Salmonella spp. were separated from fresh chicken and pork. The resistance frequency of S.aureus to all antibiotics is 0%~56.52% . 6 resistance profiles of S.aureus were found in this study. The resistance frequency of Salmonella spp. to all antibiotics is 16.67%~87.5%. There were 9 resistance profiles. Conclusion:Antibiotic resistance of S.aureus and Salmonella spp. was common in the isolates from chicken and pork in Changchun.
Key words:  S.aureus  Salmonella spp.  antibiotics resistance

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