| 摘要: |
| 应用流式细胞术(FCM)和MTS比色法检测雏鸡初次感染柔嫩艾美尔球虫(E.tenella)后外周血中CD3 CD4 、CD3 CD8 T淋巴细胞亚群和淋巴细胞增殖能力的动态变化。数据显示,E.tenella初次感染雏鸡后7-20 d CD3 CD4 、CD3 CD8 T淋巴细胞比例明显高于对照组雏鸡(p＜0.05和p＜0.01),并且均于12 d时达到峰值。初次感染后7-20 d雏鸡外周血中CD4 /CD8 T淋巴细胞亚群的比值明显升高,于感染后16 d时达到1.78。E.tenella初次感染雏鸡后12-20 d外周血淋巴细胞增殖能力明显高于对照组雏鸡(p＜0.05),并于16 d达到峰值。表明E.tenella初次感染雏鸡能激活淋巴细胞产生增殖反应,CD3 CD4 、CD3 CD8 T淋巴细胞在抵抗E.tenella初次感染过程中有重要作用。 |
| 关键词: 柔嫩艾美尔球虫 外周血 CD3 CD4 CD3 CD8 增殖 |
| DOI： |
| 投稿时间：2014-08-12修订日期：2014-08-28 |
| 基金项目:国家“863”计划项目（2013AA102806，2011AA10A215）；国家自然科学基金项目（31272552，31272541，81170358）；教育部新世纪优秀人才支持计划项目（NCET-10-0175）；吉林省科技发展计划项目（20111816）；吉林省世行贷款农产品质量安全项目（2011-Y07） |
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| Studies on Dynamic Changes of T lymphocytes subpopulations and lymphocytes proliferation in Peripheral Blood of Chickens Primary Infected with E.tenella |
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| (College of Animal Science and Technology,Jilin Agricultural University,Jilin Provincial Engineering Research Center of Animal probiotics,Changchun) |
| Abstract: |
| Flow cytometry(FCM) and MTS colorimetric assay was used to sdudy dynamic changes of T lymphocyte subpopulations and lymphocytes proliferation in peripheral blood of chicks after primary infection with E.tenella. The results were as follows: the numbers of CD3 CD4 and CD3 CD8 T lymphocytes in peripheral blood of chicks after primary infection with E.tenella were increased significantly comparing with controls after 7-20 days post infection(p＜0.05 or p＜0.01), and the |
| Key words: E.tenella peripheral blood CD3 CD4 CD3 CD8 proliferation |
