| 摘要: |
| 分别建立了清瘟败毒散超微粉中黄芩苷的薄层鉴别方法和含量测定方法。以聚酰胺层析板为薄层板，乙酸乙酯-甲酸（6:1）为展开剂，1%三氯化铁乙醇溶液为显色剂，即可快速鉴别出黄芩苷成分。高效液相色谱法的色谱条件为：色谱柱Hypersil ODS2 C18，流动相甲醇-水-磷酸(47：53：0.2)，检测波长280 nm。结果显示，黄芩苷在12~72 μg/mL范围内线性关系良好，平均回收率为99.12%。黄芩苷在清瘟败毒散超微粉中的含量相对较高，且稳定可靠，可以作为质量控制的指标。 |
| 关键词: 清瘟败毒散超微粉 黄芩苷 薄层鉴别 含量测定 |
| DOI： |
| 投稿时间：2014-09-26修订日期：2014-11-18 |
| 基金项目:河南省重点科技攻关项目（122102110185,122102110224），河南省成果转化项目（132201310002） |
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| Study on the TLC Identification and Quantitative Determination of Baicalin in Qingwen Baidusan Ultra-Fine Powder |
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| (Henan Kangxing Pharmaceutical Co.,Ltd) |
| Abstract: |
| A TLC identification method and a quantitative determination mthod were established for baicalin in Qingwen Baidusan ultra-fine powder. The polyamide chromatography plate was used, developing solvent was acetidine- formic acid(6:1) and the chromogenic agent was 1% ferric chloride ethanol solution. In this condition, baicalin was identified quckly. The HPLC methods were as follows: chromatographic column was Hypersil ODS2 C18, mobile phase was methanol - water - phosphate (47:53:0.2), and the detective wavelength was 280nm. Results showed that baicalin had good linear relationship in the range of 12~72 μg/mL, the average recovery rate was 99.12%. The content of baicalin in Qingwen Baidusan ultra-fine powder was high and stable，so it can be used as the quality index. |
| Key words: Qingwen Baidusan ultra-fine Powder baicalin TLC identification quantitative determination |
