| 摘要: |
| 为探索鸡传染性贫血重组蛋白亚单位疫苗的可行性，将鸡传染性贫血病毒基因分别克隆到转移载体pFastBacHTA中，再将其分别转化DH10Bac感受态细胞得到相应的重组表达载体，转染昆虫细胞Sf21获得含有VP1、VP2基因的重组杆状病毒vBac-VP1、vBac-VP2，应用悬浮培养的Sf21细胞表达重组蛋白。SDS-PAGE及Western blotting结果证明, VP1、VP2基因在昆虫细胞中得到了表达，动物试验进一步证实，重组蛋白免疫SPF鸡可产生ELISA抗体，这些结果表明，杆状病毒表达的VP1、VP2具有良好的免疫原性。 |
| 关键词: 鸡传染性贫血病毒 杆状病毒表达 VP1 VP2 免疫原性 |
| DOI： |
| 投稿时间：2014-10-10修订日期：2015-01-16 |
| 基金项目:科技部科技基础性工作专项“重大动物疫病病原及相关制品标准物质研究（2008FY130100）” |
|
| Expression of VP1 and VP2 genes of chicken anemia virus and Immunogenicity Research |
|
| (china institute of veterinary drug control) |
| Abstract: |
| The VP1 and VP2 genes of chicken anemia virus were cloned into pFastBacHTA vectors separately, then transform into competent cells of E. coli DH10Bac. The recombinant baculoviruses vBac-VP1 and vBac-VP2 were generated by transfection of recombinant bacmid into Sf21 insect cells. Finally, recombinant proteins were expressed in suspension culture Sf21 cells. SDS-PAGE and Western blotting showed that VP1 and VP2 have been expressed in Sf21 cells. Immunized the SPF chicken with recombinant protein, their serum were inspected by ELISA and showed good immunogenicity. |
| Key words: chicken anemia virus baculovirus expression VP1 VP2 immunogenicity |
