| 摘要: |
| 建立了3种兽药制剂中违禁药物氯丙嗪的超高效液相色谱-串联质谱（UPLC-MS/MS）检测方法。用无水乙醇超声提取试样中违禁药物氯丙嗪，采用Waters ACQUITY UPLC BEH C18柱(50 mm×2.1 mm, 1.7 μm)，乙腈和10 mmol/L乙酸铵+0.1%甲酸溶液流动相，梯度洗脱，电离喷雾电离方式（ESI+），多反应监测（MRM）定量。该方法线性关系良好，相关系数r2达到0.9956，回收率在88.3%~96.7%之间，相对标准偏差（RSD）介于3.7%～8.1%。方法的检出限为0.003 mg/kg。本方法适用于兽药制剂中氯丙嗪违法添加的定性、定量分析。 |
| 关键词: 兽药制剂 氯丙嗪 违禁药物 超高效液相色谱-串联质谱 |
| DOI： |
| 投稿时间：2015-02-10修订日期：2015-04-07 |
| 基金项目:质检行业公益性科研专项（201010073-03） |
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| Determination of Chlorpromazine Illegally Added in 3 Kinds of Veterinary Preparations by UPLC-MS/MS |
|
| (Guangdong Exit/Entry Inspection and Quarantine Bureau,Guangdong Guangzhou) |
| Abstract: |
| A method of determination of chlorpromazine illegally added in 3 kinds of veterinary preparations by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed. Chlorpromazine was extracted with ethanol by ultrasonic wave and separated on the Waters ACQUITY UPLC BEH C18 column (50 mm×2.1 mm, 1.7μm) using the mobile phase of acetonitrile/mixture of 10 mmol/L ammonium acetate and 0.1% formic acid with gradient elution. Chlorpromazine was detected in electrospray ionization (ESI ) mode using multiple reaction monitoring (MRM). There was a good linear relationship with the correlation rate more than 0.9956. The average recoveries ranged from 88.3% to 96.7%. The standard deviation was between 3.7% and 8.1%. The limit of detection was 0.003 mg/kg. The method was rapid, simple, sensitive and accurate. It was suitable for qualitative and quantitative analysis of the illicit drug chlorpromazine in veterinary preparations. |
| Key words: veterinary preparations chlorpromazine illicit drug ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) |
