| 摘要: |
| 为了探索不同理化因素对致病性维氏气单胞菌（Aeramonas veronii，AV）TH0426成膜的影响，采用改良微孔板法建立致病性AV生物膜体外模型并对其进行了研究。实验结果表明AV TH0426能够在聚乙烯酶标板表面形成生物膜，PH值4～6，10℃和NaCl浓度4g/L培养18h是形成生物膜的最适条件，黄颡鱼( Pseudobagrus fulvidraco )的肝脏提取液对AV TH0426形成生物膜有促进作用。研究结果为进一步探讨维氏气单胞菌生物膜形成机制和致病机理提供依据。 |
| 关键词: 维氏气单胞菌 形成特性 生物膜 影响因素 |
| DOI： |
| 投稿时间：2015-07-16修订日期：2015-09-09 |
| 基金项目:国家自然科学基金项目（31201927）；吉林省重点科技攻关项目（20150204065NY）；国家大学生创新创业训练计划（201410193023） |
|
| Establishment of in vitro biofilm model and influence factors of Aeramonas veronii |
|
| (College of Animal Science and Technology,Jilin Agricultural University) |
| Abstract: |
| To study the effect of different factors on bacterial biofilm formation in vitro, the modified microtiter-plate test was used to establish an in vitro biofilm model of pathogenic Aeramonas veronii TH0426 strain. The results showed that AV TH0426 could develop biofilm on the surface of the polystyrene microtiter plate, and the optimum conditions for biofilm formation of AV TH0426 strain were pH4-6, temperature 10℃,
NaCl(4g/L ), and culturing for 18h. The extract of liver increased the ability of biofilm formation by testing on the AV TH0426. |
| Key words: Aeramonas veronii (AV) characteristics biofilm physical factor |
