| 摘要: |
| 本文建立了同时测定猪粪便中二甲氧苄啶、磺胺对甲氧嘧啶、恩诺沙星、金霉素、泰乐菌素5种兽药残留量的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法。样品经McIlvaine-Na2EDTA缓冲溶液提取，C18粉吸附杂质，HLB固相萃取小柱净化，浓缩富集后用0.1%甲酸甲醇溶液复溶，过滤上机，基质液外标法定量。结果显示，5种兽药在10～500 μg/kg的质量浓度范围内线性良好，在添加浓度50~500 μg/kg，平均回收率为53%～85%，RSD为1.87%～18.81%。该方法具有较好的准确度与精密度，能快捷地测定猪粪便中5种兽药。 |
| 关键词: 超高效液相色谱-串联质谱 兽药残留 猪粪便 |
| DOI： |
| 投稿时间：2015-08-03修订日期：2015-09-21 |
| 基金项目:上海市科技兴农重点攻关项目（沪农科攻字（2014）第3-3号），公益性行业（农业）科研专项（项目编号：201203023） |
|
| Simultaneous determination of five veterinary drug residues in swine manure by ultra-performance liquid chromatography-tandem mass spectrometry |
| 顾欣 |
| (Shanghai Animal Disease Control Center) |
| Abstract: |
| A method for simultaneously detecting Diaveridine, Sulfameter, Enrofloxacin, Chlorotetracycline and Tylosin in swine manure by ultra-performance liquid chromatography-tandem mass spectrometry was developed. After having been extracted with McIlvaine-Na2EDTA buffer solution, the sample was purified and concentrated by C18 power and HLB Solid phase extraction column. The residue was dissolved by 0.1% formic acid/methanol (85/15).The results showed that five veterinary drugs were determined with a fine linear relationship from 10 to 500 μg/kg. The recoveries of five veterinary drugs spiked with 50~500 g/kg in swine manure were from 53%～85%with RSDs ranging from 1.87% to 18.81%. As a result, the method is seized of high accuracy as well as sensitivity for a rapid determination of five veterinary drugs in swine manure. |
| Key words: ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) veterinary drug residues swine manure |
