| 摘要: |
| 为探讨牛支原体氟喹诺酮类药物耐药靶位的突变情况，本试验对分离自我国多个省份的牛支原体进行氟喹诺酮类药物耐药性检测和耐药株筛选，通过对临床分离敏感株、耐药株及体外诱导高度耐药株的氟喹诺酮类药物耐药决定区（QRDR）进行测序分析，发现分离菌株中有19%（6/32株）对氟喹诺酮类药物耐药，其QRDR中均存在gyrA（Ser83Phe）或parC（Ser80Ile）的氨基酸突变；但在体外诱导的高度耐药株中QRDR突变类型则以gyrA（Ser83Phe/Tyr或Glu87Lys）和parC（Ser80Ile或Asp84Asn/Tyr）的氨基酸发生突变为主，以上靶位突变在介导牛支原体对氟喹诺酮类药物耐药水平方面是否起着决定性作用，还有待进一步证明。 |
| 关键词: 牛支原体 氟喹诺酮类药物 靶位突变 耐药机制 |
| DOI： |
| 投稿时间：2015-08-11修订日期：2015-09-23 |
| 基金项目:国家自然科学( 31272611); 吉林省科技发展计划项目任务书(20150101109JC) |
|
| Quinolones Resistance Targeted Mutation Analysis of Mycoplasma bovis |
|
| (College of Animal Science and Technology,Jilin Agricultural University) |
| Abstract: |
| In order to discuss the quinolones resistance targeted mutation analysis of Mycoplasma bovis. In?this?study,Mycoplasma?bovis??isolated?from ?several?provinces ?were?tested?on?quinolone?resistance?and quinolone resistant strains were screened.The quinolone resistance determining region(QRDR) of clinical isolates sensitive , resistant strains and resistant strains induced in vitro were sequenced.?The results showed that 19% isolates strains (6/32) were resistant to quinolones, and it was found that the resistant strains possess Ser83Phe in gyrA or ser80IleIn parC. But it found that the QRDR mutation types based on gyrA（Ser83Phe/Tyr or Glu87Lys）and parC（Ser80Ile or Asp84Asn/Tyr）in laboratory -derived resistant Mycoplasma?bovis . The above targeted mutation either whether paly an important role in mediating the quinolone resistance level of Mycoplasma bovis will remains to be further proved. |
| Key words: MycoplasmaSbovis quinolone targeted mutation resistance mechanisms |
