| 摘要: |
| 为了完善大黄末的质量控制方法,采用薄层色谱法鉴别没食子酸以及高效液相色谱法测定其含量。采用Agilent Eclipse XDB-C18 (250 mm ×4.6 mm, 5 μm)色谱柱,甲醇-0.05%磷酸溶液(5:95)为流动相,流速1.0 mL/min,检测波长为271 nm。试验结果没食子酸在1.018~101.8 μg/mL浓度范围内,线性关系良好(r= 0.9999)；平均回收率(n=6)为98.82%(RSD=0.43%)。本方法准确灵敏,可用于大黄末中没食子酸的质量控制。 |
| 关键词: 大黄末 没食子酸 薄层色谱法 高效液相色谱法 |
| DOI： |
| 投稿时间：2015-09-06修订日期：2016-01-07 |
| 基金项目: |
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| Study on the Determination and Identified of Gallic Acidin Rhubarb Powder |
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| (Zhejiang Province Institute of veterinary Drug and Feedstuff) |
| Abstract: |
| To perfect the quality control method of Rhubarb Powder. Gallic Acid was identified by TLC and determined by HPLC. A chromatographic column of Agilent Eclipse XDB-C18(250 mm ×4.6 mm, 5 μm) was used, with the mobile phase of methanol – 0.05% phosphoric acid solution (5:95) at the flow rate of 1.0 mL/min. The detection wavelength was at 271 nm . The calibration curves were linear in ranges of 1.018~101.8 μg/mL(r= 0.9999)for Gallic Acid; the average recoveries (n=6) was 98.82%(RSD=0.43%). The method is accurate and sensitive for the quality control of Rhubarb Powder. |
| Key words: Rhubarb Powder Gallic Acid TLC HPLC |
