| 摘要: |
| 从辽宁省某鸭场疑似鸭病毒性肝炎病料中分离到一株病毒，经PCR检测初步鉴定其为鸭肝炎病毒，命名为YK株，应用易感鸭胚和雏鸭进行传代及病毒含量测定，E1～E5代鸭胚适应毒病毒含量在106.5～106.9 ELD50/0.2 mL之间，E2代鸭肝组织毒病毒含量为106.3 LD50/0.1 mL。动物试验结果表明，YK株鸭胚适应毒E2代对7日龄雏鸭的致死率高达100%。序列测定分析表明，YK株与DHV-A的VP1基因同源性在71.4%～72.3%之间，与DHV-B的VP1基因同源性在71.9%～72.3%之间，与DHV-C的VP1基因同源性在94.3～98.8%之间。实验表明，YK株与韩国新型鸭肝炎病毒在同一分支上，属于基因C型DHV。 |
| 关键词: 鸭肝炎病毒 分离 鉴定 |
| DOI： |
| 投稿时间：2015-09-18修订日期：2015-12-17 |
| 基金项目: |
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| Isolation and Identification of a Duck Hepatitis Virus Strain |
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| (Liaoning Yikang Biological Co.,Ltd. Liaoyang city) |
| Abstract: |
| A virus strain was isolated from suspected duck viral disease from dead duck in liaoning Province,and identified by reverse-transcriptase polymerase chain reaction.It is a duck hepatitis virus that named YK strain.The isolates were passed from E1 to E5,the titer determination in duck embryos were from 106.5 to 106.9 ELD50/0.2 mL; the titer determination in duckling hepatitis tissue titer was 106.3/0.1mL at E2. Animal regression experimental results showed that The YK strain in duck embryos to 7- day- old duckling mortality was 100%.Sequence comparison with DHV-A serotype showed that the identity was 71.4%～72.3% , 71.9%～72.3% with DHV-B serotype and 94.3～98.8% with DHV-C serotype.It proves that the YK strain and new serotype strain in Korea belongs to DHV-C serotype. |
| Key words: duck hepatitis virus Isolation Identification |
