| 摘要: |
| 为了解不同母源抗体水平对仔猪免疫后抗体消长的影响，以及免疫后细胞因子的变化，使用口蹄疫O型灭活疫苗对40头初生仔猪进行免疫，并于初次免疫后28 d二次免疫，采用液相阻断ELISA方法检测血清中抗体滴度,双抗体夹心ELISA法检测血清IL-8和IL-12水平。结果显示，母源抗体水平越高对于初次免疫后仔猪抗体影响越大；二次免疫后抗体水平可长时间维持较高水平。初次免疫后14 d～28 d，血清中IL-8和IL-12水平上升极显著（P＜0.01）；二次免疫后14～28 d，IL-8水平上升极显著（P＜0.01），IL-12水平上升不显著（P＞0.01）。试验表明，母源抗体水平是影响仔猪免疫后抗体消长的因素之一；口蹄疫O型灭活疫苗能有效提高血清中IL-8和IL-12水平。 |
| 关键词: 口蹄疫O型灭活苗 母源抗体 白细胞介素-8 白细胞介素-12 |
| DOI： |
| 投稿时间：2016-01-16修订日期：2016-04-26 |
| 基金项目: |
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| The Effect of Type O FMD Inactivate Vaccine on Antibody and Cytokine Levels in Serum of Piglets with Different Maternal Antibodies |
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| (Inner Mongolia Agricultural University,Hohhot,;Jinyu Baoling Biology Science and Technology CoLtd) |
| Abstract: |
| In order to study the influence of different maternal antibody levels on the dynamics of antibody and the changes of cytokines after piglet immunization, type O FMD inactivate vaccine was used to immune 40 newly-born piglets, and second immunization on day 28 post primary immunity. The antibody titer in serum was detected by liquid-phase blocking ELISA, the levels of IL-8 and IL-12 in serum were detected by double antibody sandwich ELISA. Results show that the higher maternal antibody level was, the greater effect of antibody on piglets after first immunization, the antibody level can maintain a high level for a long time after booster immunity. 14 to 28 days after first immunity, the levels of IL-8 and IL-12 in serum are greatly significant (P＜0.01); 14 days to 28 days after second immunity, the level of IL-8 is greatly significant (P＜0.01); the increase of IL-12 level is not significant (P＞0.05). Results show that maternal antibody level is one of influencing factors of the dynamics of antibody after piglet immunization; type O FMD inactivate vaccine can effectively enhance the levels of IL-8 and IL-12 in serum. |
| Key words: type O FMD inactivate vaccine maternal antibody IL-8 IL-12 |
