| 摘要: |
| 建立了梯度洗脱HPLC法测定环丙氨嗪有关物质。采用Agilent Eclipse plus C18色谱柱(250 mm×4.6 mm, 5 μm),流动相A为0.02 mol/L醋酸铵溶液,流动相B为甲醇,线性梯度洗脱,流速1.0 mL/min,检测波长为213 nm。试验结果环丙氨嗪与已知5种杂质峰完全分离。本方法简单灵敏,可用于环丙氨嗪中有关物质的检测。 |
| 关键词: 环丙氨嗪 有关物质 高效液相色谱法 梯度洗脱 |
| DOI： |
| 投稿时间：2016-04-07修订日期：2016-05-06 |
| 基金项目: |
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| Gradient HPLC determination of related substances in Cyromazine |
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| (Zhejiang Province Institute of veterinary Drug and Feedstuff) |
| Abstract: |
| To develop a gradient HPLC method for the determination of related substance in cyromazine. The chromatographic column was Agilent Eclipse plus C18(250 mm ×4.6 mm, 5 μm) was used, the mobile phase consisted of 0.02 mol/L ammonium acetate (A) and methanol (B) with gradient program. The flow rate was 1.0 mL/min. The detection wavelength was at 213 nm. Good separation of cyromazine from five impurities. The method is simple and sensitive for the impurity detection of cyromazine. |
| Key words: cyromazine related substances HPLC |
