| 摘要: |
| 构建巨噬细胞验证模型,比较研究二氢槲皮素(Dihydroquercetin, also known as taxifolin, TF)和二氢杨梅素(Dihydromyricetin, DMY)体外抗炎活性。方法：以1mg/L的脂多糖(LPS)诱导RAW264.7巨噬细胞建立了体外炎症模型；通过细胞毒性试验(MTT)法检测细胞存活率；Griess试剂法测定细胞上清液中NO释放量；ELISA法检测上清液中细胞因子IL-1β、IL-6、TNF-α和PGE2的含量变化；通过RT-PCR法检测IL-1β、IL-6、TNF-α基因表达情况。MTT试验结果表明,二氢槲皮素和二氢杨梅素各剂量组没有表现出对巨噬细胞RAW264.7的细胞毒性。同时,二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能显著抑制脂多糖诱导细胞对NO、PGE2的释放(P＜0.01),且均表现出剂量依赖性；二氢槲皮素高中低剂量组和二氢杨梅素高中低剂量组均能有效降低IL-1β、IL-6、TNF-α基因的表达,同时能够降低脂多糖诱导细胞产生细胞因子IL-1β、IL-6、TNF-α的含量。二氢槲皮素和二氢杨梅素均具有良好的体外抗炎作用,且二氢槲皮素的抗炎作用较好。 |
| 关键词: 二氢槲皮素 二氢杨梅素 RAW264.7细胞 炎症 |
| DOI： |
| 投稿时间：2016-05-13修订日期：2016-06-02 |
| 基金项目:科技型中小企业创新创业(20160308002YY) |
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| Comparison of Anti-inflammatory Activity of Dihydroquercetin and Dihydromyricetin |
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| (College of Traditiongal Chinese Medicine,Jilin Agricultural University) |
| Abstract: |
| Construction of macrophages to validate the model, comparative study dihydroquercetin(also known as taxifolin, TF) and dihydromyricetin(DMY) in vitro anti-inflammatory activity. To 1mg / L lip polysaccharide (LPS) - induced RAW264.7 macrophages in vitro model of inflammation was established；Cell viability was detected by the cytotoxicity test (MTT) method；Griess reagent method was used to determine the amount of NO released from cell supernatant；The content of cytokine IL-1β, IL-6, TNF-α and PGE2 in |
| Key words: Dihydroquercetin Dihydromyricetin RAW264.7 cells inflammation |
