| 摘要: |
| 确证1批硫酸卡那霉素注射液中的非法添加物。在对该批样品进行非法添加物常规筛查时发现其含有未知色谱峰，且紫外光谱具有特征性，但与现有的紫外光谱库进行比对，无法确定该未知物的结构。结合UPLC-Q-TOF法给出的未知物的分子量信息推导出疑似添加物，再用HPLC-PDA和UPLC-Q/TOF两种方法进行验证，最终确证非法添加物。结果显示该批样品中的非法添加物为尼可刹米。通过检查该批样品中的非法添加物，总结出确证非法添加物的一般模式，从而为兽药处方外非法添加物的筛查与确证提供思路。 |
| 关键词: 尼可刹米 非法添加 |
| DOI： |
| 投稿时间：2016-06-12修订日期：2016-07-05 |
| 基金项目:“十二五”科技支撑计划（2015BAD11B03-4） |
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| Identification of Compound Illegally Adulterated in One Batch of Kanamycin Sulfate Injection by UPLC-Q/TOF MS and HPLC-PDA Method |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| To identify the unknown adulterated compound in one batch of kanamycin sulfate injection in the common screening course，an unknown compound was observed with characteristic UV spectra, and couldn’t confirm its structure．The target compound was deduced with the information provided by UPLC-Q/TOF MS and HPLC-PDA，and finally validated by the two methods above．Results showed that the unknown adulterated compound in this sample was identified as nikethamide．A model of the unknown compound identification was generalized through the progress of deduction and validation described above，which provided a reference for the future inspection of adulteration in veterinary drugs． |
| Key words: nikethamide illegal drug adulteration |
