| 摘要: |
| 为了在Marc-145细胞上获得更高滴度的猪繁殖与呼吸综合征病毒（PRRSV）TJM株，对细胞培养条件、细胞接种量、微载体的用量以及病毒培养时间等条件进行了优化。结果表明,利用生物反应器悬浮培养Marc-145细胞在血清为金源康且含量为10%、培养基为DMEM、细胞接种密度为20～30细胞/球、微载体为5 g等条件下生长状态最好；病毒最佳培养时间为27～36 h,病毒增殖效果好且能够达到最高的病毒滴度。本试验为微载体培养条件下大规模生产PRRSV-TJM株疫苗奠定了一定理论基础。 |
| 关键词: PRRSV Marc-145细胞 微载体 生物反应器 |
| DOI： |
| 投稿时间：2016-06-29修订日期：2016-09-12 |
| 基金项目:国家科技攻关计划横向课题（201500001）高致病性蓝耳病疫苗中试生产工艺及水貂犬瘟热活疫苗生物反应器悬浮培养工艺初步摸索；吉林省科技攻关计划（20150204073NY） |
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| Best Propagation Conditions of PRRSV TJM strain on Marc-145 cells in bioreactor |
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| (Institute of Special Animal And Plant Sciences of CAAS JL Teyan Biological Technology Limited Liability company) |
| Abstract: |
| In order to obtain higher titers of porcine reproductive and respiratory syndrome virus (PRRSV) TJM strain on Marc-145 cells, cell culture conditions, cell inoculation, the amount of microcarrier and time of virus culture, etc. were optimized. The results showed that the growth status of Marc-145 cells was the best by using suspension culture in the bioreactor under followed conditions: serum was Jin Yuan Kang and content was 10%; medium was DMEM; cell concentration was 20~30 cells per microcarrier; weight of microcarriers were 5 grams. Besides, when the culture time of virus was 27~36 h, the effect of virus reproduction was best and the titer was highest. This study provided a theoretical basis for large-scale production of PRRSV TJM strain vaccine. |
| Key words: PRRSV Marc-145 cell microcarrier bioreactor |
