| 摘要: |
| 为了研究热灭活、甲醛、β-丙内酯（BPL）三种不同灭活方法对新城疫病毒（NDV）血凝和RT-PCR试验结果的影响，将收获的NDV鸡胚尿囊液分别用浓度为0.1%~0.5%的甲醛37℃灭活24h或4℃灭活48h、浓度为0.02%~0.2%的BPL37℃灭活9h、60℃热灭活30~90min，并于灭活前后进行血凝试验和RT-PCR检测。结果表明，用0.02％的BPL 37℃灭活9h或0.1％的甲醛4℃灭活48h，不影响新城疫病毒的血凝价；但是，用于NDV核酸检测时，建议采用热灭活30 min，也可用0.02％的BPL 37℃灭活9h。本研究可为相关病原检测以及诊断制品制备过程中灭活方法的选择提供参考。 |
| 关键词: 新城疫病毒 灭活 血凝 RT-PCR |
| DOI： |
| 投稿时间：2016-07-23修订日期：2016-09-13 |
| 基金项目:种禽场疾病综合防控与净化技术体系评估、论证和推广研究（2016YFD0501609） |
|
| Influence of Three Different Inactivation Methods on Newcastle Disease Virus Hemagglutinin Test and RT-PCR Assay |
|
| (China Animal Health and Epidemiology Center) |
| Abstract: |
| In order to investigate the influence of different inactivation methods on Newcastle disease virus (NDV) hemagglutinin (HA) test and RT-PCR assay, viral suspensions were inactivated by 0.1%~0.5% formaldehyde for 37℃ 24h and 4℃ 48h, 0.02%~0.2% β-propiolactone (BPL) for 37℃ 9h and 60℃ water bath for 30~90min, respectively. Then, the suspensions were detected by hemagglutinin (HA) test and RT-PCR assay. The results showed that the optimal inactivation conditions for HA were 0.02％ BPL at 37℃ for 9h or 0.1％ formaldehyde at 4℃ for 48h; for RT-PCR were 60℃ water bath for 30min or 0.02％ BPL at 37℃ for 9h. This study provided suggestions for the selection of appropriate inactivation methods, which were used in detecting related pathogens and preparing diagnostic reagents. |
| Key words: Newcastle disease virus inactivation hemagglutinin RT-PCR |
