| 摘要: |
| 以提高林可霉素的生产水平为目的，将林可霉素产生菌LK15-012先用0.6%甲基磺酸乙酯(EMS)处理3 h，然后常压室温等离子体（ARTP-200W）照射90 s、最后紫外线（UV）照射50 s。结果表明：致死率为98.5%，正突变率为71.8%。最终获得2株高产突变株LK16-032和LK16-049，摇瓶效价比出发菌株LK15-012提高了42.50%和35.25%。将两株突变株分别通过100 L小试、10 t中试和60 t大生产进行发酵验证，发酵效价明显高于生产菌株。采用EMS-ARTP-UV三重复合诱变明显提高了林可霉素的生产水平。 |
| 关键词: 林可霉素 EMS-ARTP-UV三重复合诱变 高产突变株 |
| DOI： |
| 投稿时间：2016-08-04修订日期：2016-08-26 |
| 基金项目: |
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| Triple Breeding of Lincomycin Producing Strain |
|
| (Terry Pharmaceutical Co., Ltd. Ningxia Institute of bacteria) |
| Abstract: |
| In order to improve the level of production of lincomycin, clindamycin producing bacteria LK15-012 treated with 0.6% ethyl methyl sulfonic acid (EMS)3 h, the second with atmospheric pressure plasma (ARTP-200w) at room temperature irradiation of 90 s, finally by ultraviolet (UV) radiation 50 s. The results showed that the mortality rate was 98.5%, the positive mutation rate is 71.8%. Won 2 strains of high yield mutant strains LK16-032and LK16-049, shake flask titer than the starting strain LK15-012 increased by 42.50% and 35.25%.Will be high yield mutant strain respectively through the test in 100 L, 10 t pilot and 60 t fermentation carried out to verify the mass production, significantly higher than the production strains fermentation titer. The EMS-ARTP-UV triple compound mutagenesis lincomycin production levels are increased obviously. |
| Key words: lincomycin EMS-ARTP-UV mutation mutated high-producing strain |
