| 摘要: |
| 为了建立可同时检测猪肉、鸡肉组织中维吉尼霉素M1药物残留的液相色谱-串联质谱分析方法，样品选用0.2%甲酸的乙腈/水（80∶20，V/V）溶液为提取液，经Oasis PRiME HLB固相萃取小柱净化，在液相色谱-串联质谱仪上进行检测。结果表明，维吉尼霉素M1在0.25～200 ng/mL浓度范围内呈现良好的线性关系，相关系数（R2）大于0.999。猪肉、鸡肉组织中维吉尼霉素M1的检测限为0.5 μg/kg，定量限为2.0 μg/kg。在2～200 μg/kg (2MRL)添加浓度水平上，回收率范围为80% ～100%,批内、批间RSD均小于8.0%。该方法具有简便快捷、灵敏度高、定性准确等特点。 |
| 关键词: 猪肉 鸡肉 维吉尼霉素M1 兽药残留 液相色谱-串联质谱法 |
| DOI： |
| 投稿时间：2016-08-04修订日期：2017-01-04 |
| 基金项目: |
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| Determination of Virginamycin M1Resedues in Pork and Chicken by LC-MS/MS |
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| (Sichuan Institute Of Veterinary Drug Control) |
| Abstract: |
| A LC-MS/MS method was established for the determination of virginamycin M1 resedues in pork and chicken .The samples were extracted by 0.2%formate of acetonitrile-water solution (80∶20，V/V),then purified and concentrated on an Oasis PRiME HLB cartridge ,and then determined by LC-MS/MS.The results showed the good linearity in the rage of 0.25~200 ng/mL with correlation coefficients(R2) above 0.999,The limit of detection of the method was 0.5 μg/kg,and the limit of quantification was 2.0 μg/kg,The average recoveries at 2~200 μg/kg (2MRL) were 80%~100%,and intra-and inter-batch RSD were﹤8.0%.The method is simple,rapid,sensitive and accurate. |
| Key words: pork chicken virginamycin M1 veterinary residue LC-MS/MS |
