| 摘要: |
| 为研究黄连总生物碱对结核菌PhoPR双组份系统基因转录水平的影响,对不同药物作用的菌液提取总RNA并进行反转录,根据GenBank 中H37Rv的PhoP (0757) 基因、PhoR(0758) 基因和SigA的基因序列,应用Primer6.0软件设计荧光定量引物并进行荧光定量PCR分析。结果显示：在高浓度黄连生物碱作用时,多药耐药结核菌MT-7、MB-1的PhoP和PhoR的基因的表达水平明显下降,并且黄连总生物碱与RPF联合作用时,多药耐药结核菌MT-7、MB-1的PhoP和PhoR的基因的表达水平明显下降,表明黄连生物碱是RFP抗菌增效剂。研究结果为结核菌抗菌增效作用机制奠定理论基础。 |
| 关键词: 黄连总生物碱 结核菌 PhoPR 双组份系统 转录水平 |
| DOI： |
| 投稿时间：2016-08-08修订日期：2016-09-12 |
| 基金项目:吉林省科技厅重点科技攻关项目（20150204028NY）；吉林省科技厅科技成果转化促进计划（20140309018YY） |
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| Study on the Effect of Transcriptional Level on PhoPR Two-component System Genes by Coptis Chinensis Total Alkaloids |
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| (College of Chinese Medicine Materials,Jilin Agricultural University) |
| Abstract: |
| :To study on the effect of transcriptional level on PhoPR two-component system genes by Coptis chinensis total alkaloids, the different concentrations ofCoptis chinensisStotal alkaloid or inScombination with RFP were applied to detect sensitivity to MT-7, MB-1 and H37Rv. Then the total RNA was extract and reverse transcription. The expression level of PhoP and PhoR genes belonging to the PhoPR two-component system which was related to drug resistance were analyzed and compared by realtime fluores-cence quantitative PCR. The results showed that the expression level of PhoP and phoR of MT-7, MB-1 was decreased significantly with the high concentration of total alkaloids from Coptidisrhizoma. The expression of level of PhoP and phoR of MT-7and MB-1 was decreased significantly by Coptis chinensisStotal alkaloidScombined with RFP. The results showed that Coptis chinensisStotal alkaloid was Antibacterial Synerists of RFP. |
| Key words: Coptis chinensisStotal alkaloids tuberculosis bacteria PhoPR two-component system transcriptional level |
