| 摘要: |
| 目的：建立枇杷叶中齐墩果酸和熊果酸含量的高效液相色谱(HPLC)测定方法。方法：选用色谱柱Agilent Ecilpse plus C18柱(250mm×4.6mm,5μm),流动相：乙腈-0.1%氨水(70:30)磷酸调pH=8.0,流速为0.8ml/min,检测波长210nm,柱温25℃。结果：显示齐墩果酸和熊果酸能达到基线分离,分离度R>2.5,齐墩果酸在0.19854~7.9412μg、熊果酸在0.21218~8.48704μg范围内呈良好的线性关系。结论：试验证明该方法操作简单,灵敏度高,重现性好,可用于枇杷叶药材的质量控制。 |
| 关键词: 枇杷叶 齐墩果酸 熊果酸 高效液相色谱法 |
| DOI： |
| 投稿时间：2016-08-11修订日期：2016-09-29 |
| 基金项目: |
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| Determination of Oleanolic Acid and Ursolic Acid inloquat leaf by HPLC |
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| (Linzhou Sinagri Yingtai Biotech Co.Ltd) |
| Abstract: |
| Objective: To establish an HPLC method for the determination of Oleanolic Acid and Ursolic Acid in loquat leaf. Methods: HPLC was carried out on Agilent Ecilpse plus C18 column(250mm×4.6mm,5μm) at 30℃ using acetonitile-0.1%ammonium water (70:30, Phosphoric acid pH to 8.0) as mobile phase with a flow rate of 0.8 ml/min and the UV detection wavelength was set at 210 nm．Result: Oleanolic Acid and Ursolic Acid can reach the baseline separation, degreeSofSseparation(R) was more than 2.5. Oleanolic acid was linear in the range of 0.19854~7.9412μg. Ursolic acid was linear in the range of 0.21218~8.48704μg. Conclusion: The method is simple, convenient, accurate, and can be used for the quality control of loquat leaf． |
| Key words: Loquat leaf Oleanolic Acid Ursolic Acid HPLC |
