摘要: |
建立柴胡注射液、维生素C注射液、盐酸林可霉素注射液、黄芪多糖注射液中非法添加倍他米松的超高效液相色谱检查方法。采用 Waters ACQUITY UPLC ?BEH C18色谱柱(2.1×50 mm,1.7 um),以0.75%三乙胺溶液(用磷酸调节pH为3.0)-甲醇-乙腈(50:45:5)为流动相;流速为0.3 mL?min-1;二极管阵列检测器,波长采集范围为190~400 nm,记录色谱图波长242 nm。柱温25 ℃,进样量10 uL。倍他米松质量浓度在20.0~180.0 ㎎?L-1内峰面积呈良好线性关系(R2=0.999 7,n=7),在柴胡注射液、盐酸林可霉素注射液、维生素C注射液、黄芪多糖注射液中倍他米松的平均回收率分别为103.48%,RSD=0.15%(n=9);0.78%,RSD=0.78%(n=9);100.40%,RSD=1.68%(n=9);99.16%,RSD=1.74%(n=9)。本方法简便、快速、准确,可用于柴胡注射液等四种兽用注射液中非法添加倍他米松的检测。 |
关键词: 超高效液相法 柴胡注射液 维生素C注射液 盐酸林可霉素注射液 黄芪多糖注射液 倍他米松 |
DOI: |
投稿时间:2016-08-23修订日期:2017-01-04 |
基金项目: |
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Establishment of Detection Methods for betamethasone in the 4 Kinds of Veterinary Injections by UPLC-PDA |
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(Liaoning Province Animal Feed Quality and Safety of Veterinary Testing Centers) |
Abstract: |
A method for the determination of betamethasone in bupleurum injection, lincomycin hydrochloride injection, vitamin C injection, and asragulus polysacharin injection was developed by UPLC-PDA. It was tested with the Waters ACQUITY UPLC ?BEH C18 column(2.1×50 mm,1.7 um),using 0.75% tri ethylamine phosphatic liquor/Solution(pH=3.0) -methyl alcohol -acetonitrile- (50:45:5) as the mobile phase; The flow rate was 0.3ml/min; The detection wavelength was set at 242nm and simultaneously collected the spectrum at wavelengthof 190~400 nm. The column temperature had been remained in 25℃. The injection volume was 10uL. The linear range of betamethasone was 20.0-180.0 ㎎?L-1 (R2= 0.999 7,n=7) ; The mean recovery for betamethasone in bupleurum injection, lincomycin hydrochloride injection , Vitamin C Injection and asragulus polysacharin injection were 103.48%,RSD=0.15%(n=9);0.78%,RSD=0.78%(n=9);100.40%,RSD=1.68%(n=9);99.16%,RSD=1.74%(n=9). This method is accurate, easy and fast, and provides a reliable way for detecting betamethasone in bupleurum injection and another three kinds of veterinary injections. |
Key words: UPLC Bupleurum injection Vitamin C Injection Lincomycin Hydrochloride Injection Asragulus Polysacharin Injection betamethasone |