| 摘要: |
| 建立了测定利福昔明混悬乳剂含量的高效液相色谱法(HPLC)。采用C18色谱柱(4.6 ×150 mm,5 μm),以甲醇-乙腈-0.075 mol/L磷酸二氢钾溶液-1 mol/L柠檬酸溶液(32：28：25：4)为流动相,流速1.0 ml/min,检测波长236 nm,进样量20 μL,柱温25℃。利福昔明浓度在5～100 μg/mL时,其峰面积与浓度线性关系良好(r2=0.9996),无杂质干扰,稳定性高,平均回收率99.1％。本操作方法简便,分析快速,结果准确,适用于利福昔明混悬乳剂的含量测定。 |
| 关键词: 利福昔明 混悬乳剂 高效液相色谱法 含量 |
| DOI： |
| 投稿时间：2016-11-25修订日期：2017-07-03 |
| 基金项目:黑龙江省应用技术研究与开发项(czznkt2016-2) |
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| Content Determination of Rifaximin Suspo-emulsions by HPLC |
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| (Heilongjiang institute of veterinary science) |
| Abstract: |
| : Amethod for the content determination of Rifaximin suspoemulsion by HPLC was established. HPLC system consisted of C18 column(5 μm 4.6 X 150 mm), Methyl alcohol-Acetonitrile- Citric acid- Potassium Phosphate Monobasic(32：28：25：4) as mobilephase. The flow rate was 1.0 ml/min. The detection wave length was 236 nm. The injection volume was 20μl and column temperature was room temperature. There was no interference occurred from inpurities. The calibration curve was linear in the range of 5～100 μg/ml(r2=0.9996).The average recovery was 99.1％. The method is rapid, simple and accurate for the content determination of Rifaximin suspo-emulsions. |
| Key words: Rifaximin Suspo-emulsions HPLC Content |
