| 摘要: |
| 随着分子生物学的发展，16S、23S rRNA以及16S-23S rRNA已应用于菌种鉴定。16S-23S rRNA的基因间隔区相对于前两者有较高的变异性，弥补了16S和23S rRNA保守性强，分化程度不够的缺点。本研究对7株绿色气球菌和两株屎肠球菌扩增16S-23S rRNA基因间隔序列。通过随机扩增多态性DNA技术分析绿色气球菌DNA，评价不同菌株间的克隆相关性及耐药性。 |
| 关键词: 绿色气球菌 16S-23S rRNA 随机扩增多态性DNA技术 |
| DOI： |
| 投稿时间：2017-03-30修订日期：2017-10-30 |
| 基金项目: |
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| Drug Resistance Analysis of Aerococcus viridians by Random Amplified Polymorphic DNA |
|
| (College of Animal Science and Technology,Jilin Agricultural University) |
| Abstract: |
| With the development of molecular biology, the genes of 16S,23S and 16S-23S rRNA
have been used in strain identification.The gene intergenic spacer(ISR) of16S-23S rRNA has a
higher variability than the former two,which remedies the defects of the strong conservative and
the lack of differentiations of 16Sand 23S rRNA. In this study,we amplified the ISR of 7
Aerococus viridian strains and 2 Enterococcus faecium strains. We analysed the DNA of
Aerococus viridian strains and evaluated the clone correlations and resistances of different strains by randomly amplified polymorphic DNA(RAPD). |
| Key words: Aerococcus viridians 16S-23S rRNA RAPD |
