| 摘要: |
| 建立了快速测定禽蛋中氯霉素、氟苯尼考及氟苯尼考胺的超高效液相色谱-串联质谱分析方法。禽蛋用乙腈提取后，再用正己烷液液分配除脂，浓缩后用超高效液相色谱分离，以乙腈和10%甲醇水为流动相进行梯度洗脱，MRM反应监测模式检测。结果表明在5-200μg/L的系列浓度范围内均呈良好线性关系，相关系数均大于0.999。氯霉素、氟苯尼考、氟苯尼考胺的检测限为0.5μg/kg，定量限为2.0 μg/kg，本方法在2μg/kg～20μg/kg添加浓度范围内平均回收率为60%～100%，精密度小于20%。该方法方便快捷、定性准确适于禽蛋中的氯霉素、氟苯尼考及氟苯尼考胺药物残留检测。 |
| 关键词: 禽蛋 氯霉素类药物 残留 超高效液相色谱-串联质谱 |
| DOI： |
| 投稿时间：2017-06-20修订日期：2017-11-20 |
| 基金项目: |
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| Rapid Determination of Chloramphenicol，Florfenicol and its metabolites in poultry Eggs by UPLC-MS/MS |
|
| (Hubei Institute of Veterinary Drug Control) |
| Abstract: |
| Abstract :A UPLC-MS/MS method was developed and established to rapidly determine chloramphenicol, florfenicol and florfenicol-amine in poultry eggs. The eggs were extracted with acetonitrile and then separated by n-hexane liquid. After enrichment, the samples were separated by UPLC, elutedwith acetonitrile and 10% methanol as the mobile phase and the MRM reaction was monitored. The results showed that there was a good linearity in the range of 5-200μg/L ,and the correlation coefficients were more than 0.999. The detection limit of the chloramphenicol, florfenicol and florfenicol-amine was 0.5μg/kg, and the limit of quantification was 2μg/kg. The average recoveries were 60% ~ 100% in the range of 2μg/kg to 20μg/kg, and the precision was less than 20%. The method was convenient, rapid, accurate and it was suitable for the detection of chloramphenicol in poultry eggs. |
| Key words: poultry eggs chloramphenicol residues UPLC-MS/MS |
