| 摘要: |
| 建立并验证了刺五加甘草颗粒中紫丁香苷含量的测定方法。采用高效液相色谱法，色谱柱为Agilent Zorbax SB-C18（250 × 4.6 mm，5 μm），流动相为乙腈-水梯度洗脱；检测波长为265 nm；流速为1.0 ml/min；柱温为30 ℃。试验结果表明紫丁香苷进样浓度在0.01218 ~ 0.08120 mg/ml范围内，与峰面积呈良好的线性关系（r = 0.9999），加样回收率在96.46% ~ 99.53%之间，平均回收率为98.4%（n=6），RSD为1.2%,。所建立的方法简便、准确度高、专属性强，可用于刺五加甘草颗粒中紫丁香苷含量的测定。 |
| 关键词: 刺五加 紫丁香苷 高效液相色谱法 含量 甘草 |
| DOI： |
| 投稿时间：2018-06-11修订日期：2018-09-27 |
| 基金项目: |
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| Determination of Syringin in Acanthopanax Glycyrrhiza granules by HPLC |
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| (Luoyang Huizhong Animal Medicine Co,Ltd) |
| Abstract: |
| The method for determination of the content of syringin in Acanthopanax Glycyrrhiza granules by HPLC was developed and validated. The analysis was performed on an Agilent Zorbax SB-C18 column(250×4.6 mm, 5 μm). The gradient mobile phase was consisted of acetonitrile-water, and the detection wavelength was 265 nm. The flow rate was 1.0 mL/min and the column temperature was 30 ℃. The linear range of the calibration curves for syringin was 0.01218 ~ 0.08120 mg/ml (r = 0.9999), and the average recovery was 98.4% (n=6) with RSD of 1.2%. The method is simple and accurate with high specificity, which can be used to determine the content of syringin in Acanthopanax Glycyrrhiza granules. |
| Key words: Acanthopanax syringin HPLC content Glycyrrhiza |
