| 摘要: |
| 以鱼腥草芩蓝口服液为研究对象，摸索不同流动相、溶剂、柱温、流速条件下的色谱图，通过中药指纹图谱相似度软件评价系统，计算相似度等相关参数，最终确定一个HPLC指纹图谱条件：Thermo C18色谱柱（250 mm×4.6 mm，5 μm），以甲醇（A）-1 %磷酸水溶液（B）为流动相梯度洗脱，柱温25 ℃，体积流量1.0 mL·min-1，检测波长为240 nm。本研究建立的方法可靠，简便易行，为全面控制鱼腥草芩蓝口服液的质量提供科学依据。 |
| 关键词: 鱼腥草芩蓝口服液 HPLC 指纹图谱 黄芩苷 绿原酸 连翘苷 精氨酸 |
| DOI： |
| 投稿时间：2018-07-04修订日期：2018-12-05 |
| 基金项目:石家庄市科学技术研究与发展计划项目(编号：186500067A,名称：现代中药鱼腥草芩蓝口服液新兽药的研发)。 |
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| Studies on HPLC Fingerprint of Yuxingcaoqinlan oral liquid |
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| (1. Hebei Weierli Animal Pharmaceutical Group Co.,Ltd. Shijiazhuang 050200;China; 2. Hebei province functional feed additive engineering technology research center. Shijiazhuang 050200 China; 3. Shijiazhuang Animal Functional Additive Engineering Technology Research Center,Shijiazhuang 050200;China.) |
| Abstract: |
| Taking the oral liquid of Yuxingcaoqinlan as the research object, The chromatograms of different mobile phases, solvents, column temperatures and flow rates were explored, Similarity evaluation for chromatographic fingerprint of traditional Chinses medicine was used for data analysis. Finally, a HPLC fingerprint condition was obtained: Thermo C18 column（250 mm×4.6 mm, 5 μm）, the mobile phase composed of methanol (A) and 1% phosphoric acid aqueous solution (B) in gradient elution. The column temperature was maintained at 25℃, The flow rate was 1.0mL·min-1 and detection wavelength was set at 240nm. The established method is simple and reliable, and the HPLC fingerprint can be used for the quality control of the oral liquid of Yuxingcaoqinlan. |
| Key words: Yuxingcaoqinlan oral liquid HPLC Fingerprint Baicalin Chlorogenic acid Phillyrin Arginine |
