| 摘要: |
| 为了测定清肺止咳散中黄芩苷的含量，采用C18柱（5 μm，250 mm×4.6 mm），在室温下，以甲醇-0.3%磷酸溶液（55：45）为流动相，以276 nm为检测波长，流速为1.0 ml/ min，柱温30℃，建立了高效液相色谱测定方法。黄芩苷在3.8～379 μg/ml范围内线性良好, R2 =0.9998,平均回收率为94.3%（n=6），RSD为0.92%。本方法简便、准确、可靠，可用于控制清肺止咳散中黄芩苷的含量。 |
| 关键词: 高效液相色谱法 清肺止咳散 黄芩苷 |
| DOI： |
| 投稿时间：2019-01-02修订日期：2019-05-30 |
| 基金项目: |
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| The content of baicalin in Qingfei Zhike Powder was determined by HPLC |
|
| (Shaanxi Provincial Veterinary Drug Monitoring Institute) |
| Abstract: |
| To determine the content of Baicalin in Qingfei Zhike Powder. Using C18 column（5 μm 250mm×4.6mm），At room temperature, methanol-0.3% phosphoric acid solution (55:45) was used as the mobile phase, The detection wavelength was 276 nm, The flow rate of 1.0 ml/min,The temperature of column is 30℃.The results showed, Baicalin had a good linear relationship in 3.8～379.0 in the range of igg /ml, R2 =0.9998, The average recovery rate was 94.3% and RSD 0.92%. This method is convenient accurate and fast, and can be used to control the content of scutellaria baicalensis in Qingfei Zhike Powder. |
| Key words: HPLC Qingfei Zhike Powder Baicalin |
