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高效液相色谱法测定七清败毒颗粒中黄芩苷含量的方法研究
侯丽丽,刘春龙
0
(山西省兽药监察所;江西新世纪民星动物保健品有限公司)
摘要:
为完善七清败毒颗粒质量标准,采用高液相色谱法对七清败毒颗粒中黄芩苷的含量测定进行了研究。色谱柱为C18(4.6mm×250mm,5μm),流动相为甲醇-水-磷酸(41:59:0.2),检测波长为280nm,流速为1ml/min,柱温30℃,进样量10μl。黄芩苷进样浓度在0.005~0.12mg/ml范围内,峰面积与黄芩苷含量呈良好的线性关系(r=0.9991),样品平均回收率为99.2%(n=6),RSD为1.04%。本方法简便、准确度高、重复性好,为进一步控制七清败毒颗粒的质量标准提供了依据。
关键词:  高效液相色谱法  七清败毒颗粒  黄芩苷
DOI:
投稿时间:2019-02-26修订日期:2019-08-20
基金项目:
Determination of Baicalin in Qiqingbaidu Particle by HPLC
(shanxi Institute of veterinary Drug Control)
Abstract:
In order to improve the quality standard of Qiqingbaidu Baicalin,an HPLC method for the content determination of baicalin in Qiqingbaidu particle was developed.The isolation was performed on a C18 column (4.6mm×250mm,5μm)with mobile phase consisting of methyl alcohol-water-phosphoric acid (41:59:0.2),The detection wavelength was 280nm,The flow rate was 1ml/min,The column temperature was 30℃,the injection volume was 10μl,There was a good linear correlation between the peak areas and baicalin the concentrations of baicalin at the range of 0.005~0.12mg/mL(r=0.9991) by HPLC.The average recovery was 99.2% with RSD od 1.04%(n=60,The results indicated that the method was simple,accurate and repeatable ,It can be used for the quality control of Qiqingbaidu Particle.
Key words:  HPLC  Qiqingbaidu particle  baicalin

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