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高效液相色谱法同时测定兽用制剂中氟苯尼考、糠酸莫米松、盐酸特比萘芬的含量
张璐,于晓辉
0
(中国兽医药品监察所)
摘要:
建立了同时测定兽用制剂中氟苯尼考、糠酸莫米松、盐酸特比萘芬含量的高效液相色谱法。C8(4.6 mm×250 mm,5 μm)色谱柱分离,甲醇-0.02%三乙胺水溶液(75∶25)为流动相,流速为1.0 mL/min,采用224 nm作为氟苯尼考及盐酸特比萘芬的检测波长、250nm作为糠酸莫米松的检测波长分别进行检测,。氟苯尼考、糠酸莫米松、盐酸特比萘芬线性范围分别为0.49~49.20 μg/mL(r=1.000)、0.24~7.96 μg/mL(r=1.000)、0.25~50.00 μg/mL(r=1.000),其回收率(n=9)分别为100.80%、 102.83%、101.37%。测得兽用制剂中氟苯尼考、糠酸莫米松、盐酸特比萘芬含量分别为15.46 mg/mL、2.07 mg/mL、15.35 mg/mL。结果表明该方法可用于兽用制剂中氟苯尼考、糠酸莫米松及盐酸特比萘芬的含量的测定。
关键词:  兽用制剂  氟苯尼考  糠酸莫米松  盐酸特比萘芬  高效液相色谱法
DOI:
投稿时间:2019-05-12修订日期:2019-08-28
基金项目:
Simultaneous Determination of Florfenicol,Mometasone Furoate and Terbinafine Hydrochloride in Veterinary Preparations by HPLC
(China Institute of Veterinary Drugs Control,Beijing 10081,China)
Abstract:
This research was aimed to establish an HPLC method for the simultaneous determination of florfenicol, mometasone furoate and terbinafine hydrochloride in veterinary preparations. A Thermo C8 column (4.6 mm×250 mm, 5 μm) was adopted as analytical column. The mobile phase consisted of methanol-0.02% triethylamine (75∶25) at a flow rate of 1.0 mL/min, the detection wavelengths were 224 nm for florfenicol and terbinafine hydrochloride, 250 nm for mometasone furoate, and the injection volume was 10 μL. The linear ranges observed were 0.49~49.20 μg/mL (r=1.000), 0.24~7.96 μg/mL (r=1.000) and 0.25~50.00 μg/mL (r=1.000). The average recoveries (n=9) were 100.80%, 102.83% and 101.37%. The contents of florfenicol, mometasone furoate and terbinafine hydrochloride in a veterinary preparation were 15.46 mg/mL, 2.07 mg/mL, 15.35 mg/mL. The method can be used for simultaneous determination of florfenicol, mometasone furoate and terbinafine hydrochloride in veterinary preparations.
Key words:  veterinary preparations  florfenicol  mometasone furoate  terbinafine hydrochloride  HPLC

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