| 摘要: |
| 目的 为了考察穿梅三黄散超微粉和细粉的显微特征及药效区别。方法 通过生物显微镜和扫描电镜对超微粉和细粉显微特征进行观察,通过体外抑菌试验和饲喂试验对药效进行考察。结果 穿梅三黄散经超微粉碎后,粉末粒径更小,失去常见的显微鉴别特征；超微粉对3种水产致病菌(嗜水气单胞菌、柱状黄杆菌和肠型点状气单胞菌)的最小抑菌浓度(MIC)均小于细粉；穿梅三黄散能够降低试验草鱼血清中AST、ALT酶活性及肝组织中MDA含量,提高试验草鱼肝组织中CAT、SOD及GSH-PX酶活性,且作用效果优于细粉(p＜0.05或p＜0.01)。结论 穿梅三黄散超微粉能够显著提高抑菌能力及鱼类肝脏抗损伤能力。 |
| 关键词: 穿梅三黄散 超微粉 显微特征 最小抑菌浓度（MIC） 肝脏抗损伤能力 |
| DOI： |
| 投稿时间：2019-07-17修订日期：2019-11-21 |
| 基金项目:武汉市农科院财政示范项目(Czsf201803） |
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| Comparative Study on the Microscopic Characteristics and Efficacy between Fine and Ultrafine Powder of Chuan mei san huang San |
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| (Wuhan Chopper Fishery Bio-Tech Co,Ltd;Wuhan Institute of fishery) |
| Abstract: |
| Object: The study was aimed to compare the microscopic characteristics and efficacy between fine and ultrafine powder of Chuan mei san huang San. Methods: The microscopic characteristics were observed with microscope and scanning electron microscope. The efficacy were investigated by vitro antibacterial experiment and feeding experiment. Results: The powder size of ultrafine powder was smaller and the common characteristics of microscopic identification disappeared. Ultrafine powder had smaller MIC on Aeromonas hydrophila, Flavobacterium cloumnare and AromonasSpunctata f.intestinalis. The activities of ALT, AST and the level of MDA were decreased significantly, and the activities of SOD, CAT, GSH-Px were increased significantly compared with the blank control group(P＜0.05 or＜0.01), and ultrafine powder had better effects than the fine powder. Conclusion: Ultrafine powder of Chuan mei san huang San could improve the antibacterial activity and protection of hepatic tissue of fish. |
| Key words: Chuan mei san huang San ultrafine powder microscopic characteristic MIC protection of hepatic tissue |
