引用本文
  •    [点击复制]
  •    [点击复制]
【打印本页】 【下载PDF全文】 查看/发表评论下载PDF阅读器关闭

←前一篇|后一篇→

过刊浏览    高级检索

本文已被:浏览 117次   下载 83 本文二维码信息
码上扫一扫!
MDCK细胞悬浮驯化及对H9亚型禽流感病毒敏感性的研究
习硕,赵蕾,史爱华,章振华,李林,沈佳,崔丽娜,姜北宇,张建伟
0
(北京市农林科学院畜牧兽医研究所 100097)
摘要:
为了获得对H9亚型禽流感病毒敏感的纯悬浮MDCK细胞株,通过逐渐降低血清的方法对贴壁培养的MDCK细胞进行了的无血清纯悬浮驯化,并最终获得了一株对H9亚型禽流感敏感的纯悬浮的细胞株MDCK-sus。通过测定表明,该细胞株能够迅速增殖,倍增时间为24 h,使用H9亚型禽流感病毒BX13株感染该细胞,培养72 h,培养液的病毒HA价可达到1:512,每0.1 mL病毒含量达到108.5 EID50,为利用生物反应器大规模悬浮培养制备H9亚型禽流感病毒抗原奠定了基础。
关键词:  MDCK悬浮细胞  禽流感病毒  敏感性
DOI:
投稿时间:2019-07-23修订日期:2019-09-18
基金项目:北京市农林科学院畜牧兽医研究所公益院所基金(XMS201908)
Conversion of MDCK cell line to suspension culture and its sensitivity study to H9 subtype avian influenza virus
(Beijing Academy of Agriculture and Forestry Sciences)
Abstract:
In order to obtain all suspension MDCK cell line sensitive to H9 subtype avian influenza virus, the adherent MDCK cells were domesticated in serum-free suspension by gradually decreasing serum levels and a pure suspension cell line MDCK-sus susceptible to H9 subtype avian influenza was finally obtained. The results indicate that the cell line can multiply rapidly with a doubling time of 24 hours. After 72 hours of incubation with H9 subtype avian influenza virus BX13 strain, the virus HA of the culture medium can reach 1:512, and the viral content can reach 108.5 EID50 every 0.1 mL, which laid a foundation for the production of H9 subtype avian influenza virus antigen by large-scale suspension culture in bioreactor.
Key words:  MDCK suspension cell  influenza virus  sensitivity

用微信扫一扫

用微信扫一扫