| 摘要: |
| 为了测定山苓健胃颗粒中甘草酸的含量。采用C18柱（5 μm，250 mm×4.6 mm），在室温下，以甲醇-0.2 mol/L醋酸铵溶液-冰醋酸（65﹕35﹕1，V/V）为流动相，以250 nm为检测波长，流速为1.0 ml/min，建立了高效液相色谱测定方法。结果表明甘草酸在3.274～49.11 μg/ml范围内线性关系良好r =0.9996平均回收率为99.9%，RSD为1.30%。本方法准确、简便、快速，可用于控制山苓健胃颗粒中甘草酸的含量。 |
| 关键词: 山苓健胃颗粒 高效液相色谱法 甘草酸 |
| DOI： |
| 投稿时间：2019-08-06修订日期：2019-10-18 |
| 基金项目: |
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| The content of glycyrrhizic acid in Shanlingjianwei Granule was determined by HPLC |
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| (Sichuan Institute of Veterinary Drug Control) |
| Abstract: |
| The content of glycyrrhizic in Shanlingjianwei granules was determined. Using C18 column（5 μm，250 mm×4.6 mm）at room temperature, methanol-0.2mol/L ammonium acetate solution- glacial acetic acid (65﹕35﹕1, V/V) was used as the mobile phase, the detection wavelength was 250nm, the flow rate of 1.0 ml/min, a HPLC method for determination was established.The results showed, glycyrrhizic acid had a good linear relationship in 3.274 ~ 49.11 in the range of μg/ml, r=0.9996, the average recovery rate was 99.9% and RSD 1.30%. This method is accurate, simple and fast, and can be used to control the content of glycyrrhizic acid in Shanlingjianwei granules. |
| Key words: Shanlingjianwei Granule high performance liquid chromatography(HPLC) glycyrrhizic acid |
