| 摘要: |
| 建立同时快速检测牛奶中环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星六种氟喹诺酮类药物残留的酶联免疫吸附法。ELISA法的操作在20℃~25℃下进行,以50%抑制浓度、最低检测限反映方法的灵敏度,同时进行加标回收试验。结果表明,本文所建立的ELISA法的最低检测限为1.48μg/L,50%抑制浓度的范围在0.254~0.361μg/L；在环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、氧氟沙星和洛美沙星药物添加浓度为5μg/L、25μg/L、50μg/L时的回收率为84.58%~115.92%,批内变异系数均小于15.0%,批间变异系数均小于16.0%。本方法简便快捷,灵敏度、准确度和精密度高,适用于牛奶中FQs残留的同时快速检测。 |
| 关键词: 酶联免疫吸附法 牛奶 氟喹诺酮类药物 残留 |
| DOI： |
| 投稿时间：2019-09-03修订日期：2019-12-24 |
| 基金项目:四川省科技计划（2019YFS00620），四川大学高校基本科研业务费研究专项项目（skqy201234） |
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| Determination of Fluoroquinolones Residues in Milk by Enzyme-linked Immunosorbent Assay |
|
| (West China School of Public Health and West China Fourth Hospital,Sichuan University) |
| Abstract: |
| An enzyme-linked immunosorbent assay was established for the simultaneous determination of six fluoroquinolone residues in milk, includes ciprofloxacin, enoxacin, norfloxacin, pefloxacin, ofloxacin and lomefloxacin. ELISA was performed at 20℃~25℃, the sensitivity of the method was reflected by 50% inhibition concentration and minimum detection limit, the recovery test was carried out at the same time. The results showed that the minimum detection limit of this method was 1.48 μg/L, the range of 50% inhibitory concentration was 0.254~0.361μg/L; The recovery rate of ciprofloxacin, enoxacin, norfloxacin, pefloxacin, ofloxacin and lomefloxacin at 5μg/L, 25μg/L and 50μg/L was 84.58%~115.92%, the intra-batch variation coefficient was less than 15.0%, and the inter-batch variation coefficient was less than 16.0%. This method is simple and rapid, with high sensitivity, accuracy and precision. It is suitable for the rapid screening and detection of the residues of fluoroquinolones in milk. |
| Key words: ELISA milk fluoroquinolones residues |
