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| 高效液相色谱法测定微球中硫酸小檗碱的含量 |
| 朱庆贺,王丽坤,张艳,王爽,陈曦,王观悦,穆永才,罗天瑶,史同瑞 |
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| (黑龙江省农业科学院畜牧兽医分院) |
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| 摘要: |
| 建立了测定微球中硫酸小檗碱含量的高效液相色谱法(HPLC)。采用C18色谱柱(4.6 × 250 mm,5 μm),以乙腈-水(45：55)为流动相,流速1.0 mL/min,检测波长349 nm,进样量5 μL,柱温30℃。硫酸小檗碱浓度在0.1～50 μg/mL时,其峰面积与浓度线性关系良好(r2=0.9991),无杂质干扰,稳定性高,平均回收率98.98％。本操作方法简便,分析快速,结果准确,适用于微球中硫酸小檗碱的含量测定。 |
| 关键词: 硫酸小檗碱 微球 高效液相色谱法 含量 |
| DOI： |
| 投稿时间：2019-11-06修订日期：2020-01-14 |
| 基金项目:黑龙江省应用技术研究与开发项(czznkt2018-2) |
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| Content Determination of Berberine sulfuric Microspheres by HPLC |
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| (Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang) |
| Abstract: |
| A method for the content determination of Berberine sulfuric Microspheres by HPLC was established. HPLC system consisted of C18 column(5 μm 4.6 X 250 mm), Methyl alcohol-water(45:55) as mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 349 nm. The injection volume was 5 μL and column temperature was 30 ℃. There was no interference occurred from inpurities. The calibration curve was linear in the range of 0.098～49.00 μg/mL（r2=0.9991）.The average recovery was 98.98％. The method is rapid, simple and accurate for the content determination of berberine sulfuric suspo-emulsions. |
| Key words: Berberine sulfuric Microspheres HPLC Content |
