| 摘要: |
| 为研究蒲公英提取物联合多种药物对奶牛乳房炎金黄色葡萄球菌的体外协同抑菌效果，以期应用于临床，减少抗菌药的使用量，降低细菌耐药性和兽药残留。试验选取3种来源（河北，东北，北京）的蒲公英提取物，通过高效液相色谱仪对3种不同来源的蒲公英提取物的主成分及含量进行测定并分析，选取后期试验所需的蒲公英提取物来源。并应用微量肉汤稀释法测定多种抗生素对金黄色葡萄球菌的MIC值（最小抑菌浓度），随后测试蒲公英提取物与抗菌药物联合用药，是否对金黄色葡萄球菌具有协同效果。试验结果表明，不同来源的蒲公英提取物主成分含量差异较大，含有单咖啡酰酒石酸、咖啡酸、菊苣酸、绿原酸，但含量比例不同。随之测得不同来源蒲公英提取物对金黄色葡萄球菌的敏感程度也有差异，临床用药前有必要进行药敏试验科学精准的选择更合适的药物。蒲公英提取物与金霉素、恩诺沙星、阿莫西林、氨苄西林、头孢噻呋、头孢喹肟等联合用药，对金葡菌的体外抑菌实验有良好的协同作用，体外能显著降低抗菌药物的用量。 |
| 关键词: 蒲公英提取物 高效液相色谱法 含量测定 最小抑菌浓度 联合用药 |
| DOI： |
| 投稿时间：2019-11-24修订日期：2020-02-25 |
| 基金项目:国家科技攻关计划2015年国家科技支撑计划“兽药安全与质量评价技术研究与应用”编号“BAD11B03-01” |
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| In vitro synergistic antibacterial effect of dandelion extract combined with antibiotics on Staphylococcus aureus |
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| (Institute of Veterinary Drug Quality Inspection of Shandong Province) |
| Abstract: |
| In order to study the in vitro synergistic antibacterial effect of dandelion extract combined with several drugs on Staphylococcus aureus of mastitis in dairy cows, in order to reduce the use of antibiotics, bacterial resistance and veterinary drug residues. The main components and contents of Dandelion extracts from three different sources (Hebei, northeast, Beijing) were determined and analyzed by HPLC. The mic value (minimum inhibitory concentration) of several antibiotics to Staphylococcus aureus was measured by broth dilution method, and then the synergistic effect of dandelion extract and antibiotics on Staphylococcus aureus was tested. The results showed that the main components of Dandelion extracts from different sources were different, including caffeic acid, tartaric acid, chicory acid and chlorogenic acid, but the content proportion was different. The sensitivity of Dandelion extracts from different sources to Staphylococcus aureus is also different. It is necessary to carry out drug sensitivity test before clinical use to select more appropriate drugs scientifically and accurately. The combination of dandelion extract with aureomycin, enrofloxacin, amoxicillin, ampicillin, ceftiofur and cefquinoxime has a good synergistic effect on the in vitro antibacterial experiment of Staphylococcus aureus, and can significantly reduce the dosage of antibacterial drugs in vitro. |
| Key words: Dandelion extract HPLC Content determination Minimal inhibitory concentration Drug combination |
