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多拉菌素的复合诱变及发酵条件优化
张会萍,张萍,牛春
0
(宁夏泰瑞制药股份有限公司)
摘要:
为通过诱变获得遗传性状稳定的多拉菌素高产菌株,对多拉菌素产生菌RDL19-1进行多种诱变处理包括常压室温等离子(ARTP)诱变,紫外(UV)诱变,及ARTP-UV复合诱变。该菌株接种瓶后于28 ℃摇床培养32 h,以10%的接种量转入装量为35 mL/300 mL 发酵瓶中,在28 ℃条件下发酵培养14 d,多拉菌素发酵产量达到了1390 μg/mL,相较于诱变前提高了4倍。通过对多拉菌素产生菌RDL19-1进行ARTP-UV复合诱变,获得了一株遗传性状稳定的高产菌株。
关键词:  多拉菌素  复合诱变
DOI:
投稿时间:2020-08-20修订日期:2021-03-31
基金项目:
Compound Mutagenesis of Doramectin and Optimization of Fermentation Conditions
(Ningxia Tairui Pharmactlal Co.,Ltd)
Abstract:
To obtain a high-yield doramectin strain with stable genetic traits by mutagenesis, the doramectin-producing strain RDL19-1 was subjected to various mutagenesis treatments such as atmospheric room temperature plasma (ARTP) mutagenesis, ultraviolet (UV) mutagenesis, and ARTP-UV combined mutagenesis. After inoculating the flask, the strain was cultured on a shaker at 28 ℃ for 32 hours, and transferred to a 35 mL/300 mL fermentation flask with 10% of the inoculum, and fermented and cultured at 28 ℃ for 14 days. The fermentation yield of doramectin reached 1390 μg/mL, which is 4 times higher than that before mutagenesis. By ARTP-UV compound mutagenesis of doramectin-producing strain RDL19-1, a high-yielding strain with stable genetic characteristics was obtained.
Key words:  doramectin  compound mutagenesis

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