| 摘要: |
| 为通过诱变获得遗传性状稳定的多拉菌素高产菌株，对多拉菌素产生菌RDL19-1进行多种诱变处理包括常压室温等离子(ARTP)诱变，紫外(UV)诱变，及ARTP-UV复合诱变。该菌株接种瓶后于28 ℃摇床培养32 h，以10%的接种量转入装量为35 mL/300 mL 发酵瓶中，在28 ℃条件下发酵培养14 d，多拉菌素发酵产量达到了1390 μg/mL，相较于诱变前提高了4倍。通过对多拉菌素产生菌RDL19-1进行ARTP-UV复合诱变，获得了一株遗传性状稳定的高产菌株。 |
| 关键词: 多拉菌素 复合诱变 |
| DOI： |
| 投稿时间：2020-08-20修订日期：2021-03-31 |
| 基金项目: |
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| Compound Mutagenesis of Doramectin and Optimization of Fermentation Conditions |
|
| (Ningxia Tairui Pharmactlal Co.,Ltd) |
| Abstract: |
| To obtain a high-yield doramectin strain with stable genetic traits by mutagenesis, the doramectin-producing strain RDL19-1 was subjected to various mutagenesis treatments such as atmospheric room temperature plasma (ARTP) mutagenesis, ultraviolet (UV) mutagenesis, and ARTP-UV combined mutagenesis. After inoculating the flask, the strain was cultured on a shaker at 28 ℃ for 32 hours, and transferred to a 35 mL/300 mL fermentation flask with 10% of the inoculum, and fermented and cultured at 28 ℃ for 14 days. The fermentation yield of doramectin reached 1390 μg/mL, which is 4 times higher than that before mutagenesis. By ARTP-UV compound mutagenesis of doramectin-producing strain RDL19-1, a high-yielding strain with stable genetic characteristics was obtained. |
| Key words: doramectin compound mutagenesis |
