| 摘要: |
| 采用UPLC-PDA联合UPLC-MS/MS方法确证了未知药品中的卡那霉素。样品经提取稀释后，采用ACQUITY UPLC T3色谱柱为分离柱，UPLC-PDA初步筛查，再用正离子扫描，液相色谱串联质谱仪上机测定。结果显示，未知物确证是卡那霉素；样品检出限为0.1 μg/mL，定量限为0.2 μg/mL。本方法快速、灵敏、重现性好，适用于此批次未知药品中卡那霉素的检测。 |
| 关键词: 卡那霉素 未知药品 超高效液相-串联质谱法 |
| DOI： |
| 投稿时间：2021-01-08修订日期：2021-05-28 |
| 基金项目:2015年国家科技支撑计划“兽药安全与质量评价技术研究与应用”（BAD11B03-01） |
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| UPLC-PDA combined UPLC-MS/MS method to confirm kanamycin in a batch of unknown drugs |
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| (Shandong Provincial Veterinary Medicine Supervision Institute,Jinan) |
| Abstract: |
| UPLC-PDA combined UPLC-MS/MS method was used to confirm kanamycin in unknown drugs. The sample was extracted and diluted, and the ACQUITY UPLC T3 chromatographic column was used as the separation column, UPLC-PDA preliminary screening, then positive ion scanning, liquid chromatography series mass spectrometer on the machine. The results showed that the unknown substance was kanamycin, the detection limit was 0.1μg/mL, the quantitative limit was 0.2μg/mL. This method is rapid, sensitive and reproducible, and is suitable for the detection of kanamycin in this batch of unknown drugs. |
| Key words: Kanamycin Unknown drugs UPLC-MS/MS |
