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| 伊维菌素纳米乳中伊维菌素含量HPLC检测方法的建立 |
| 苗艳,朱庆贺,陈曦,杨旭东,王爽,王丽坤,陈亮,兰世捷,冯万宇,李丹,史同瑞 |
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| (黑龙江省农业科学院畜牧兽医分院) |
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| 摘要: |
| 建立了伊维菌素纳米乳中伊维菌素含量的高效液相色谱检测方法(HPLC)。采用C18色谱柱(4.6 × 250 mm，5 μm)，以甲醇-乙腈-水(40∶55∶5)为流动相，流速1.0 mL/min，检测波长254 nm，进样量10 μL，柱温25 ℃。伊维菌素浓度在4.6～92.0 μg/mL时，其峰面积与浓度线性关系良好(r2=0.9996)，回收率高，平均回收率为98.25%~98.76%。精密度良好，RSD均小于2%，色谱峰图无杂质干扰，稳定性高，本操作方法简便，分析快速，结果准确，适用于伊维菌素纳米乳剂的含量测定。 |
| 关键词: 伊维菌素 纳米乳剂 高效液相色谱 含量 |
| DOI： |
| 投稿时间：2021-01-09修订日期：2021-07-05 |
| 基金项目:黑龙江科研机构创新能力提升专项“兽药新药新品种开发”(YC2016D004)；“农业科技创新跨越工程”专项(HNK2019CX15-10)；农业科技创新跨越工程”专项(HNK2019CX16) |
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| Content Determination of Ivermectin Nano-emulsion by HPLC |
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| (Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences) |
| Abstract: |
| A method for the content determination of ivermectin nano-emulsion by HPLC was established. HPLC system consisted of C18 column(4.6×250 mm，5 μm), methyl alcohol-acetonitrile-water(40∶55∶5) as mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 254 nm. The injection volume was 10 μL and column temperature was room temperature. There was no interference occurred from inpurities. The calibration curve was linear in the range of 4.6～92.0 μg/mL(r2=0.9996).The average recovery was 98.25%~98.76%. The method is rapid, simple and accurate for the content determination of ivermectin nano-emulsion. |
| Key words: ivermectin nano-emulsion HPLC content |
